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MgO Nanomaterials Improve Fibroblast Adhesion and Proliferation

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Abstract

Magnesium (Mg) plays an important role in the body mediating cell-extracellular matrix (ECM) interactions, bone apatite structure and density, and nucleic acid chemistries. While Mg has been investigated as a biomaterial for bone applications, it has not been studied for applications within soft tissues. This study investigated, for the first time, the response of fibroblasts to magnesium oxide (MgO) nanoparticles for soft tissue engineering applications. Primary human dermal fibroblasts were cultured both on tissue culture polystyrene in media supplemented with MgO nanoparticles as well as on poly-L-lactic acid (PLLA)-MgO nanoparticle composites. As this study was conducted concurrently with a study aimed at bone tissue engineering, hydroxyapatite (HA) nanomaterials were used for comparison. Results showed for the first time that fibroblasts adhered onto MgO-containing composites roughly three times better than HA-PLLA samples and roughly 4.5 times better than plain PLLA samples. Fibroblasts also proliferated to statistically higher densities when cultured in medium supplemented with MgO nanoparticles compared to un-supplemented medium and medium supplemented with HA nanoparticles. These preliminary results together suggest that MgO nanoparticles should be further investigated as materials to improve the regeneration of soft tissues as well as bone.

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References

  1. Smith L, Thomopoulos S. Tendon/Ligament-to-Bone Tissue Engineering - Current and Emerging Strategies. US Musculoskeletal Review 2011;6:11–5.

    Google Scholar 

  2. Maguire ME, Cowan JA. Magnesium chemistry and biochemistry. Biometals 2002;15(3):2013–210.

    Google Scholar 

  3. Webster PO. Magnesium. American Journal of Clinical Nutrition 1987;45:1305–1312.

    Article  Google Scholar 

  4. Bigi A, Foresti E, Gregorini R, Ripamonti A, Roveri N, Shah JS. The role of magnesium on the structure of biological apatites. Calcif. Tissue Int. 1992;50:439–444.

    Article  CAS  Google Scholar 

  5. Tsuboi S, Nakagaki H, Ishiguro K, Kondo K, Mukai M, Robinson C, Weatherell JA. Magnesium distribution in human bone. Calcif. Tissue Int. 1992;50:34–37.

    Google Scholar 

  6. Serre CM, Papillard M, Chavassieux P, Voegel JC, Boivin G. Influence of magnesium substitution on a collagen-apatite biomaterial on the production of a calcifying matrix by human osteoblasts. J Biomed Mater Res. 1998;42:626–633.

    Article  CAS  Google Scholar 

  7. Zreiqat H, Howlett CR, Zannettino A, Evans P, Schulze-Tanzil G, Knabe C, Shakibaei M. Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants. Journal of Biomedical Materials Research 2002;62:175–184.

    Article  CAS  Google Scholar 

  8. Yamniuk AP, Vogel HJ. Calcium- and magnesium-dependent interactions between calcium- and integrin-binding protein and the integrin αIIb cytoplasmic domain. Protein Science 2005;14(6):1429–1437.

    Article  CAS  Google Scholar 

  9. Gupta B, Revagade N, Hilborn J. Poly(lactic acid) fiber: An overview. Progress in Polymer Science 2007;32:455–82.

    Article  CAS  Google Scholar 

  10. Li W, Cooper Jr J, Mauck RL, Tuan RS. Fabrication and characterization of six electrospun poly(alpha-hydroxy ester)-based fibrous scaffolds for tissue engineering applications. Acta Biomaterialia 2006;2:377–85.

    Article  Google Scholar 

  11. Sui G, Yang X, Mei F, Hu X, Chen G, Deng X, Ryu S. Poly-L-lactic acid/hydroxyapatite hybrid membrane for bone tissue regeneration. J Biomed Mater Res A 2007;82:445–54.

    Article  Google Scholar 

  12. Liu H, Webster TJ. Mechanical properties of dispersed ceramic nanoparticles in polymer composites for orthopedic applications. International Journal of Nanomedicine 2010;2010:299–313.

    Google Scholar 

  13. Lee JB, Park HN, Ko WK, Bae MS, Heo DN, Yang DH, Kwon IK. Poly(L-lactic acid)/hydroxyapatite nanocylinders as nanofibrous structure for bone tissue engineering scaffolds. J Biomed Nanotechnol 2013;9:424–29.

    Article  CAS  Google Scholar 

  14. Denkena B, Lucas A, Thorey F, Waizy H, Angrisani N, Meyer-Lindenberg A. Biocompatible magnesium alloys as degradable implant materials - Machining induced surface and subsurface properties and implant performance. Special Issues on Magnesium Alloys 2011.

    Google Scholar 

  15. Weng L, Webster TJ. Nanostructured magnesium increases bone cell density. Nanotechnology 2012;23(48).

    Google Scholar 

  16. Lopez-Macipe A, Rodriguez-Clemente R, Hidalgo-Lopez A, Arita I, Garcia-Garduno MV, Rivera E, Castano VM. Wet chemical synthesis of hydroxyapatite particles from nonstoichiometric solutions. J Mater Synth Process 1998;6:21–26.

    Article  CAS  Google Scholar 

  17. Hickey DJ, Webster TJ. Adding MgO nanoparticles to hydroxyapatite-PLLA nanocomposites for improved bone tissue engineering applications. Submitted.

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Acknowledgments

The authors gratefully thank Mr. Robert Egan and Mr. William Fowle for their technical assistance, the Northeastern University Department of Chemical Engineering for facilities, and NSF-IGERT Grant No. 0965843 for funding.

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Authors and Affiliations

  1. Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, U.S.A.

    Daniel J. Hickey & Thomas J. Webster

  2. Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia

    Thomas J. Webster

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  1. Daniel J. Hickey
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  2. Thomas J. Webster
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Hickey, D.J., Webster, T.J. MgO Nanomaterials Improve Fibroblast Adhesion and Proliferation. MRS Online Proceedings Library 1722, 26–31 (2014). https://doi.org/10.1557/opl.2015.438

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  • DOI: https://doi.org/10.1557/opl.2015.438

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