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A novel, green, and biocompatible graphene-based carbonaceous material for immobilization of cytochrome c

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Abstract

The immobilization of cytochrome c (cyt c) on tea polyphenol functionalized and reduced graphene oxide (TPG) was carried out by a simple adsorption process. Intriguingly, TPG with large surface area exhibited excellent adsorption behaviors and good biocompatibility. The adsorbed materials were characterized by various methods including scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). And the effects of adsorption behavior of cyt c were discussed in detail. The results showed the adsorption behavior was dependent on the pH value and showed a high adsorption capacity as high as 1.414 × 104 mg/g and was friendly to normal cells (mouse fibroblast cell line, L929). In conclusion, we proposed the introduction of TPG as novel material and used the adsorption method to immobilize cyt c, which would provide a novel material and simple method for the enrichment of protein.

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ACKNOWLEDGMENT

This work was supported by National Natural Science Foundation of China (81400534).

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People’s Republic of China

    Kuo Gai, Qian Huang, Sainan Zheng, Lei Zhang, Chaoliang Zhang & Liying Hao

  2. Department of Mining Engineering, Shanxi Institute of Energy, Taiyuan, 030600, People’s Republic of China

    Maoping Kang

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  1. Kuo Gai
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  2. Maoping Kang
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  3. Qian Huang
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  4. Sainan Zheng
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  5. Lei Zhang
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  6. Chaoliang Zhang
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  7. Liying Hao
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Correspondence to Liying Hao.

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Gai, K., Kang, M., Huang, Q. et al. A novel, green, and biocompatible graphene-based carbonaceous material for immobilization of cytochrome c. Journal of Materials Research 33, 4270–4277 (2018). https://doi.org/10.1557/jmr.2018.387

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  • DOI: https://doi.org/10.1557/jmr.2018.387

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