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Effect of Ti6Al4V surface morphology on the osteogenic differentiation of human embryonic stem cells

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Abstract

Ti6Al4V alloys usually need to undergo some kind of surface treatment to enable good bone growth and implant integration. In this work, three treatments that modify the titanium alloy surface were studied with the aim of promoting osteogenic differentiation of human-embryonic-stem-cell-derived mesenchymal progenitors (hESC-MPs). The surface treatments used were mechanical polishing and electropolishing for 4 or 12 min in an H2SO4/HF/glycerine solution. The samples were characterised by atomic force microscopy, profilometry, X-ray photoelectron spectroscopy, and wettability. Samples were seeded with hESC-MPs in osteogenic media, and the cell number and alkaline phosphatase activity were measured. The electropolishing surface treatments influenced the nanometric morphology and wettability. However, the electropolished surfaces contributed in the same way as the mechanically polished surface to osteogenic differentiation, indicating that differentiation was strongly influenced by microroughness, which did not differ among the treatments used in the present work.

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ACKNOWLEDGMENTS

The present work was carried out with the support of CAPES and CNPq, Brazilian Government entities focused on the formation of human resources. We thank Julie Marshall from the University of Sheffield for technical services relating to the cell culture work. The authors also thank INPE for the XPS analysis.

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Authors and Affiliations

  1. Laboratório de Pesquisa em Corrosão/Programa de Pós-Graduação em Engenharia de Minas, Metalúrgica e de Materiais (LAPEC/PPGE3M), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, 91501-970, Brasil

    Leonardo Marasca Antonini, Vinícius Kothe & Célia de Fraga Malfatti

  2. Institute for in silico Medicine (INSIGNEO), University of Sheffield, Sheffield, S1 3JD, UK

    Gwendolen C. Reilly & Robert Owen

  3. Instituto Nacional de Pesquisas Espaciais (INPE), São José dos Campos, SP, 12228-970, Brasil

    Jossano Saldanha Marcuzzo

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  1. Leonardo Marasca Antonini
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  2. Vinícius Kothe
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Correspondence to Leonardo Marasca Antonini.

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Antonini, L.M., Kothe, V., Reilly, G.C. et al. Effect of Ti6Al4V surface morphology on the osteogenic differentiation of human embryonic stem cells. Journal of Materials Research 32, 3811–3821 (2017). https://doi.org/10.1557/jmr.2017.392

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