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Epidemiological and genetic characterization of larval stages of Fasciola gigantica in snail intermediate hosts in Karnataka State, India

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Abstract

Fasciolosis in ruminants in India is caused by the liver fluke Fasciola gigantica. Radix (Lymnaea) spp. are known to carry the infective stages of this parasite. Understanding the seasonal prevalence of F. gigantica infection in the intermediate host is of extreme importance in order to elucidate the transmission dynamics of the parasite. So the present study was designed to determine the bioclimatic distribution of larval stages of F. gigantica in Radix spp. snails as well as to explore the genetic diversity of F. gigantica in three geographical regions (Deccan plateau, Western Ghats and coastal region) of Karnataka. The lymnaeid snails were sampled (n = 2077) for a period of one year (June 2015 to May 2016) at 24 sites. The snails were morphologically identified and the infection status was established through cercarial shedding and nested polymerase chain reaction (PCR) based technique targeting second internal transcribed spacers (ITS-2) of nuclear ribosomal DNA. The sensitivity of PCR (8.2%) for detection of F. gigantica infection within snail is significantly higher than cercarial shedding (4.3%) with an overall prevalence of 5.1%. The prevalence of infection was higher in winter than in the rainy and summer seasons (6.2% instead of 4.6% and 4.3% respectively). Deccan plateau (5.8%) showed a higher prevalence of infection compared to Western Ghats (5.2%) and Coastal region (3.6%). The sequencing ITS-2 region permitted the identification of the parasite as F. gigantica which is having high implication in studying the population genetic structure of the parasite in the country. In conclusion, overall results indicated that Radix spp. snails harboured F. gigantica developmental stages throughout the year and nested PCR was found to be sensitive and specific for detection of F. gigantica infection in snails compared to routine parasitological techniques.

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References

  • Amor N, Farjallah S., Salem M., Lamine D.M., Paolo M., Said K., et al. 2011. Molecular characterization of Fasciola gigantica from Mauritania based on mitochondrial and nuclear ribosomal DNA sequences. Experimental Parasitology, 30, 1–10. DOI: 10.1016/j.exppara.2011.07.002

    Google Scholar 

  • Bardhan D., Kumar R.R., Nigam C.S., Mishra H., Bhoj S. 2014. Estimation of milk losses due to fasciolosis in Uttarakhand. Agricultural Economics Research Review, 27, 281–288. DOI: 10.5958/0974-0279.2014.00031.7

    Article  Google Scholar 

  • Bargues M.D., Mas-Coma S. 1997. Phylogenetic analysis of lymnaeid snails based on 18S rDNA sequences. Molecular Biology and Evolution, 14, 569–577. DOI: 10.1093/oxfordjournals.molbev.a025794

    Article  CAS  Google Scholar 

  • Bargues M.D., Artigas P., Khoubbane M., Ortiz P., Naquira C, Mas-Coma S. 2012. Molecular characterization of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis. Parasites and Vectors, 5, 174. DOI: 10.1186/1756-3305-5-174

    Article  CAS  Google Scholar 

  • Bauri R. 2015. Epidemiological studies on some important trematodes of domestic ruminants in snail intermediate hosts in Urtar Pradesh. Thesis submitted to the Deemed University, Indian Veterinary Research Institute, Izatnagar — 243 122 (U.P), India

    Google Scholar 

  • Charlier J., Duchateau L., Claerebout E., Williams D., Vercruysse J. 2007. Associations between anti-Fasciola hepatica antibody levels in bulk-tank milk samples and production parameters in dairy herds. Preventive Veterinary Medicine, 78, 57–66

    Article  Google Scholar 

  • Dung B.T., Doanh P.N., The D.T., Loan H.T., Losson B., Caron Y. 2013. Morphological and molecular characterization of lymnaeid snails and their potential role in the transmission of Fasciola spp. in Vietnam. Korean Journal of Parasitology, 51. 657–662. DOI: 10.3347/kjp.2013.51.6.657

    Article  Google Scholar 

  • Gloer P. 2002. Uberfamilie Lymnaeoidea Rafinesque 1815. Familie Lymnaeid ae Lamarck 1812. In: Glöer P. (ed.) Die Süßwassergastropoden Nord-und Mitteleuropas. Bestimmungschlüssel, Lebensweise, Verbreitung. Die TierweltDeutschlands 73. Conchbooks, Hackenheim, pp. 200–232. (In German)

    Google Scholar 

  • Hillis D.M., Dixon M.T. 1991. Ribosomal DNA: Molecular evolution and phylogenetic inference. The Quarterly Review of Biology, 66, 411–453. DOI: 10.1086/417338

    Article  CAS  Google Scholar 

  • Hope Cawdery M.J., Strickland K.L., Conway A., Crow P.J. 1977. Production effects of liver fluke in cattle. The effects of infection on live weight gain, food intake and food conversion efficiency in beef cattle. British Veterinary Journal, 133, 145–59

    Article  Google Scholar 

  • Islam Z., Alam M.Z., Akter S., Roy B.C., Mondai M.M.H. 2012. Distribution patterns of vector snails and trematode cercaria in their vectors in some selected areas of Mymensingh. Journal of Environmental Science and Natural Resources, 5, 37–46. DOI: 10.3329/jesnr.v5i2.14599

    Article  Google Scholar 

  • Jouet D., Ferte H., Depaquit J., Rudolfova J., Latour P., Zanella D., et al. 2008. Trichobilharzia spp. in natural conditions in Annecy Lake, France. Parasitology Research, 103, 51–58. DOI: 10.1007/s00436-008-0926-3

    Article  Google Scholar 

  • Lyholt H.C.K., Buchmann K. 1996. Diplostomum spathaceum: effects of temperature and light on cercarial shedding and infection of rainbow trout. Diseases of Aquatic Organisms, 25, 169–173. DOI:10.3354/dao025169

    Article  Google Scholar 

  • Malek A.E., Cheng T.C. (Ed.). 1974. Medical and Economic Malacology. Academic Press, London, pp. 1–398

    Google Scholar 

  • Mehra U.R., Verma A.K., Dass R.S., Sharma R.L., Yadav S.C. 1999. Effects of Fasciola gigantica infection on growth and nutrient utilization of buffalo calves. Veterinary Research, 145: 699–702

    CAS  Google Scholar 

  • Nilsson C., Ericsson U., Medin M., Sundberg I. 1998. Freshwater snails in south Sweden — compared with 1940 — century- Environmental Protection Agency, Report 4903

    Google Scholar 

  • Oakley G.A., Owen B., Knapp N.H.H. 1979. Production effects of subclinical liver fluke infection in growing dairy heifers. Veterinary Record, 104: 503–507

    Article  CAS  Google Scholar 

  • Raina O.K., Jacob S.S., Sankar M., Bhattacharya D., Bandyopadhyay S., Varghese A., etal. 2015. Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers. Journal of Parasitic Diseases, 39, 27–32. DOI: 10.1007/s12639-013-0276-7

    Article  CAS  Google Scholar 

  • Rao N.V.S. (Ed.). 1989. Hand book of fresh water molluscs of India. Zoological Survey of India, Calcutta, India, pp. 1–289

    Google Scholar 

  • Remigio E.A. 2002. Molecular phylogenetic relationships in the aquatic snail genus Lymnaea, the intermediate host of fhe causative agent of fascioliasis: insights from broader taxon sampling. Parasitology Research, 88, 687–696. DOI: 10.1007/s00436-002-0658-8

    Article  CAS  Google Scholar 

  • Rondelaud D., Titi A., Vignoles P., Mekroud A., Dreyfuss G. 2013. Consequence of temperature changes on cercarial shedding from Galba truncatula infected with Fasciola hepatica or Paramphistomum daubneyi. Parasite, 20, 1–7. DOI: 10.1051/parasite/2013009

    Article  Google Scholar 

  • Ross J.G 1970. The economics of Fasciola hepatica infections in cattle. British Veterinary Journal, 126, 13–15

    Google Scholar 

  • Schweizer G., Braun U., Deplazes P., Torgerson P.R. 2005. Estimating the fînancial losses due to bovine fasciolosis in Switzerland. Veterinary Record, 157, 188–193. DOI: 10.1136/vr.157.7.188

    Article  CAS  Google Scholar 

  • Sierra R.M.Y., Artigas P., Cuervo P., Deis E., Sidoti L., Mas Coma S., et al. 2009. Fascioliasis transmission by Lymnaea neotropica confirmed by nuclear rDNA and mtDNA sequencing in Argentina. Veterinary Parasitology, 166, 73–79. DOI: 10.1016/j.vetpar.2009.08.001

    Article  CAS  Google Scholar 

  • Snedecor G.W, Cochran W.G (Sixth Ed.). 1968. Statistical Methods Iowa State University Press, Ames

  • Spithill T.W., Smooker P.M., Copeman D.B. 1999. Fasciola gigantica: epidemiology, control, immunology and molecular Biology In: Dalton J.P. (Ed.) Fasciolosis. CABI Publishing; Oxon, UK, 465–525

    Google Scholar 

  • Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  Google Scholar 

  • Vassilev G.D., Jooste R.L. 1991. Production losses and control of fasciolosis in cattle in Zimbabwe. Zimbabwe Veterinary Journal, 22, 45–56

    Google Scholar 

  • Yadav C.L., Vatsya S., Garg R., Kumar R.R., Banerjee P.S., Rajesh G. 2007. Seasonal prevalence of Fasciola gigantica infection in sheep and goats in western Uttar Pradesh. Journal of Parasitic Diseases, 31, 141–144

    Google Scholar 

  • Yakhchali M., Viayeh R.M., Imani-Baran A. 2014. PCR-RFLP analysis of 28S rDNA for specification of Fasciola gigantica (Cobbold, 1855) in the infected Lymnaea auricularia (Linnaeus, 1785) snails from northwestern Iran. Iranian Journal of Parasitology, 9, 358–364

    PubMed  PubMed Central  Google Scholar 

  • Yakhchali M., Viayeh R.M., Imani Baran A.2015. Molecular detection of the infection with Fasciola hepatica in field-collected snails of Galba truncatula and Lymnaea stagnalis from West Azarbaijan. Iran Archives of Razi Institute, 70, 195–202. DOI: 10.7508/ARI.2015.03.008

    Google Scholar 

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Authors and Affiliations

  1. Centre for Advanced Faculty Training, Veterinary, College, Hebbal, Bengaluru, Karnataka, India, 560024

    Rangappa Rajanna, Pamalapati Mahadevareddy Thimmareddy & Gotakanapura Sanjeevamurthy Mamatha

  2. ICAR- National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Karnataka, India, 560064

    Pinaki Prasad Sengupta & Susan Jacob Siju

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  1. Rangappa Rajanna
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  2. Pamalapati Mahadevareddy Thimmareddy
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  3. Pinaki Prasad Sengupta
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  5. Gotakanapura Sanjeevamurthy Mamatha
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Correspondence to Pinaki Prasad Sengupta.

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Rajanna, R., Thimmareddy, P.M., Sengupta, P.P. et al. Epidemiological and genetic characterization of larval stages of Fasciola gigantica in snail intermediate hosts in Karnataka State, India. Acta Parasit. 63, 609–616 (2018). https://doi.org/10.1515/ap-2018-0070

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  • DOI: https://doi.org/10.1515/ap-2018-0070

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