Skip to main content
SpringerLink
Log in

In SituHybridization

  • Protocol
Medical Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 1996 Accesses

Abstruct

In situ hybridization is a method used to localize nucleic acid sequences in cells or tissue sections. The first in situ hybridization experiments were described by two groups of researchers in 1969, John et al. and Gall and Pardue (reviewed in ref. 1). Radioactively labeled RNA probes were used to localize specific DNA sequences within the nuclei of Xenopus laevis (2).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Warford, A. (1994) An overview of in situ hybridisation, in A Guide to In Situ Hybaid, UK, pp. 5–17.

    Google Scholar 

  2. John, H. A, Birnstiel, M. L., and Jones, K. W. (1969) RNA-DNA hybrids at the cytological level. Nature 223, 582–587.

    Article  PubMed  CAS  Google Scholar 

  3. Herrington, C. S. (1998) Demystified...in situ hybridisation. J. Clin. Pathol: Mol. Pathol. 51, 8–13.

    Article  CAS  Google Scholar 

  4. Williams, C., Ponten, F., Moberg, C., et al (1999) A high frequency of sequence alterations is due to formalin fixation of archival specimens. Am. J. Pathol. 155, 1467–1471.

    Article  PubMed  CAS  Google Scholar 

  5. Hopman, A. H. N., Ramaekers, F. C. S., Raap, A. K., et al. (1988) In situ hybridization as a tool to study numerical chromosome aberrations in solid bladder tumors. Histochemistry 89, 307–316.

    Article  PubMed  CAS  Google Scholar 

  6. Hopman, A. H. N., van Hooren, E., van de Kaa, C. A., Vooijs, P. G. P., and Ramaekers, F. C. S. (1991) Detection of numerical chromosome aberrations using in situ hybridization in paraffin sections of routinely processed bladder cancers. Mod. Pathol. 4, 503–512.

    PubMed  CAS  Google Scholar 

  7. Sauter, G., Moch, H., Wagner, U., et al (1995) Y chromosome loss detected by FISH in bladder cancer. Cancer Genet. Cytogenet. 82, 163–169.

    Article  PubMed  CAS  Google Scholar 

  8. Murphy, D. S., McHardy P., Coutts, J.. et al. (1995) Interphase cytogenetic analysis of erbB2 and topoII? co-amplification in invasive breast cancer and polysomy of chromosome 17 in ductal carcinoma in situ. Int. J. Cancer 64, 18–26.

    Article  CAS  Google Scholar 

  9. Wolf, N. G., Abdul-Karim, F. W., Schork, N. J., and Schwartz, S. (1996) Origins of heterogeneous ovarian carcinomas. A molecular cytogenetic analysis of histologically benign, low malignant potential, and fully malignant components. Am. J. Pathol. 149, 511–520.

    PubMed  CAS  Google Scholar 

  10. Lawrence Fox, J., Hsu, P-H., Legator, M., Morrison, L. E., and Seelig, S. A. (1995) Fluorescence in situ hybridisation: powerful molecular tool for cancer progniosis. Clin. Chem. 41, 1554–1559.

    Google Scholar 

  11. McNicol, A. M. and Farquharson, M. (1997) In situ hybridisation and its diagnostic applications in pathology. J. Pathol. 182, 250–261.

    Article  PubMed  CAS  Google Scholar 

  12. Poulsom, R. (2001) In situ hybridisation to localise mRNAs, in Metastasis Methods & Protocols (Brooks, S. A. and Schumacher, U., eds.), Humana, Totowa, NJ, pp. 177–198.

    Chapter  Google Scholar 

  13. Poetsch, M., Dittberner, T., Woenckhaus, C., and Kleist, B. (2000) Use of interphase cytogenetics in demonstrating specific chromosomal aberrations in solid tumors-new insights in the pathogenesis of malignant melanoma and head and neck squamous cell carcinoma. Histol. Histopathol. 15, 1225–1231.

    PubMed  CAS  Google Scholar 

  14. Herrington, C. S. and McGee, J. O.'D.(1992) Principles and basic methodology of DNA/RNA detection by in situ hybridisation, in Diagnostic Molecular Pathology, A Practical Approach, Volume I (Herrington, C. S. and McGee, J. O.'D, eds.), Oxford University Press, Oxford.

    Google Scholar 

  15. Bartlett, J. M. S., Watters, A. D., Going, J. J., Ballantyne, S. A., Grigor, K. M., and Cooke, T. G. (1998) Quantitative FISH: is chromosome 9 loss a marker of disease recurrence in TCC of the bladder? Br. J. Cancer 77, 2193–2198.

    Article  PubMed  CAS  Google Scholar 

  16. Watters, A. D., Ballantyne, S. A., Going, J. J., Grigor, K. M., Cooke, T. G., and Bartlett, J. M. S. (2000) Aneusomy of chromosomes 7 & 17 predicts recurrence of transitional cell carcinoma of the urinary bladder. Br. J. Urol. 85, 42–47.

    CAS  Google Scholar 

  17. Watters, A. D. and Bartlett, J. M. S. (2000) Quantitation of FISH signals in archival tumours, in Ovarian Cancer: Methods and Protocols (Bartlett, J. M. S., ed.), Humana, Totowa, NJ, Vol. 3, pp. 253–260.

    Chapter  Google Scholar 

  18. Syrjanen, S. (1992) Viral gene detection by in situ hybridisation, in Diagnostic Molecular Pathology, A Practical Approach, Volume I (Herrington, C. S. and McGee, J. O'D., eds.), Oxford University Press, Oxford.

    Google Scholar 

  19. Hopwood, D. (1996) Fixation and fixatives, in Theory and Practice of Histological Techniques, 4th ed., (ai]Bancroft, J. and Stevens, A., eds.), Churchill Livingstone, London.

    Google Scholar 

  20. McKenzie, K. J., Ferrier, R. K., Howatson, A. G., and Lindop, G. B. M. (1996) Demonstration of renin gene expression in nephroblastoma by in situ hybridisation. J. Pathol. 180, 71–73.

    Article  PubMed  CAS  Google Scholar 

  21. Watters, A. D. and Bartlett, J. M. S. (2002) Fluorescence in situ hybridization in paraffin tissue sections: pretreatment protocol. Mol. Biotechnol. 21, 217–220.

    Article  PubMed  CAS  Google Scholar 

  22. Leitch, A. R., Schwarzacher, T., Jackson, D., and Leitch, I. J. (1994) In Situ Hybridisation. BIOS Scientific, Oxford.

    Google Scholar 

  23. Mitchell, B. S., Dhami, D., and Schumacher, U. (1992) In situ hybridisation: a review of methodologies and applications in the biomedical sciences. Med. Lab. Sci. 49, 107–118.

    PubMed  CAS  Google Scholar 

  24. Roche Diagnostics (2002) DIG Application Manual for Non-Radioactive In Situ Hybridisation, 3rd ed. Roche Diagnostics.

    Google Scholar 

  25. Arber, D. A. (2000) Molecular diagnostic approach to non-Hodgkin’s lymphoma. J. Mol. Diagn. 2, 178–190.

    Article  PubMed  CAS  Google Scholar 

  26. Pauletti, G., Godolphin, W., Press, M. F., and Slamon, D. J. (1996) Detection and quantitation of HER-2/neu gene amplification in human breast cancer archival material using fluorescence in situ hybridization. Oncogene 13, 63–72.

    PubMed  CAS  Google Scholar 

  27. Haralambieva, E., Kleiverda, K., Mason, D. Y., Schuuring, E., and Kluin, P. M. (2002) Detection of three common translocation breakpoints in non-Hodgkin’s lymphomas by fluorescence in situ hybridisation on routine paraffin-embedded tissue sections. J. Pathol. 198, 163–170.

    Article  PubMed  CAS  Google Scholar 

  28. Nomura K., Yoshino, T., Nakamura, S., et al (2003) Detection of t(11;18)(q21;q21) in marginal zone lymphoma of mucosa-associated lymphocytic tissue type on paraffin-embedded tissue sections by using fluorescence in situ hybridisation. Cancer Genet. Cytogenet. 140, 49–54.

    Article  PubMed  CAS  Google Scholar 

  29. Reese, D. M. and Slamon, D. J. (1997) HER2/neu signal transduction in human breast and ovarian cancer. Stem Cells 15, 1–8.

    Article  PubMed  CAS  Google Scholar 

  30. Zujewski, J. O. (2003) “Build quality in”-HER2 testing in the real world. JNCI 94, 788–789.

    Google Scholar 

  31. Rhodes, A., Jasani, B., Couturier, J., et al (2002) A formalin-fixed, paraffin-processed cell line standard for quality control of immunohistochemical assay of HER2/neu expression in breast cancer. Am. J. Clin. Pathol. 117, 81–89.

    Article  PubMed  CAS  Google Scholar 

  32. Herrington, C. S., Worsham, M., Southern, S. A., et al. (1996) Loss of chromosome 9 in tissue sections of transitional cell carcinomas as detected by interphase cytogenetics. A comparison with RFLP analysis. J. Pathol. 179, 169–176.

    Article  Google Scholar 

  33. Aldosari, N., Bigner, S. H., Burger, P. C., et al. (2002) MYCC and MYCN oncogene amplification in medulloblastoma: a fluorescence in situ hybridisation study on paraffin sections from the children’s oncology group. Arch. Lab. Med. 126, 540–544.

    Google Scholar 

  34. Halling, K. C., King, W., Sokolova, I. A., et al. (2000) A comparison of cytology and fluorescence in situ hybridisation for the detection of urothelial carcinoma. J. Pathol. 164, 1768–1775.

    CAS  Google Scholar 

  35. Lamar Jones, M. (2002) Molecular pathology and in situ hybridisation, in Theory and Practice of Histological Techniques, 5th ed., (Bancroft, J. D. and Gamble, M., eds.), Churchill Livingston, London.

    Google Scholar 

  36. Unger, E. R. and Vernon, S. D. (2001) Detection of human papillomaviruses by polymerase chain reaction and in situ hybridisation, in Methods in Molecular Medicine (Killeen, A. A.,ed.), Humana, Totowa, NJ.

    Google Scholar 

  37. Ramaekers, F. C. S. and Hopman, A. H. N. (1993) Detection of genetic aberrations in bladder cancer using in situ hybridization. Ann. NY Acad. Sci. 677, 199–213.

    Article  PubMed  CAS  Google Scholar 

  38. Poddighe, P. J., Bringuier, P-P., Vallinga, M., Schalken, J. A., Ramaekers, F. C. S., and Hopman, A. H. N. (1996) Loss of chromosome 9 in tissue sections of transitional cell carcinomas as detected by interphase cytogenetics. A comparison with RFLP analysis. J. Pathol. 179, 169–176.

    Article  PubMed  CAS  Google Scholar 

  39. Russell, A. J., Sibbald, J., Haak, H., Keith, W. N., and McNicol, A. M. (1999) Increasing genomic instability in adrenocortical carcinoma progression with involvement of chromosomes 3, 9, and X at the adenoma stage. Br. J. Cancer 81, 684–89.

    Article  PubMed  CAS  Google Scholar 

  40. Speel, E. J. M., Jansen, M. P. H. M., Ramaekers, F. C. S. and Hopman, A. H. N. (1994) A novel triple-color detection procedure for brightfield microscopy, combining in situ hybridization with immunocytochemistry. J. Histochem. Cytochem. 42, 1299–1307.

    PubMed  CAS  Google Scholar 

  41. Botti, C., Pescatore, B., Mottolese, M., et al. (2000) Incidence of chromosomes 1 and 17 aneusomy in breast cancer and adjacent tissue: an interphase cytogenetic study. J. Am. Coll. Surg. 190, 516–525.

    Article  Google Scholar 

  42. Micale, M. A., Visscher, D. W., Gulino, S. E., and Wolman, S. R. (1994) Chromosomal aneuploidy in proliferative breast disease. Hum. Pathol. 25, 29–35.

    Article  PubMed  CAS  Google Scholar 

  43. Steidl, C., Simon, R., Bürger, H., et al. (2002) Patterns of chromosomal aberrations in urinary bladder tumours and adjacent urothelium. J. Pathol. 198, 115–120.

    Article  PubMed  Google Scholar 

  44. Bubendorf, L., Nocito, A., Moch, H., and Sauter, G. (2001) Tissue microarray (TMA) technology: minaturized pathology archives for high-throughput in situ studies. J. Pathol. 195, 72–79.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Western Infirmary, Glasgow, Scotland, UK

    Amanda D. Watters

Authors
  1. Amanda D. Watters
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker  & Ralph Rapley  & 

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Watters, A.D. (2005). In SituHybridization. In: Walker, J.M., Rapley, R. (eds) Medical Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-870-6:409

Download citation

  • DOI: https://doi.org/10.1385/1-59259-870-6:409

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-288-9

  • Online ISBN: 978-1-59259-870-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation