Medical Biomethods Handbook

  • John M. Walker
  • Ralph Rapley

Table of contents

  1. Front Matter
    Pages i-xv
  2. Ralph Rapley
    Pages 1-12
  3. Marilena Aquino de Muro
    Pages 13-23
  4. Gareth J. S. Jenkins
    Pages 25-34
  5. Bronwen Harvey, Pirkko Soundy
    Pages 35-42
  6. Pirkko Soundy, Bronwen Harvey
    Pages 43-62
  7. Jeroen H. Roelfsema, Dorien J. M. Peters
    Pages 79-86
  8. Anu Suomalainen, Ann-Christine Syvänen
    Pages 87-94
  9. Beatriz Sanchez-Vega
    Pages 95-116
  10. Simon G. Gregory
    Pages 117-133
  11. Ioannis Bossis, Antonios Voutetakis, Constantine A. Stratakis
    Pages 135-145
  12. Marian Hill
    Pages 147-153
  13. Richard Kitching, Arun Seth
    Pages 155-165
  14. Carla Osiowy
    Pages 167-181
  15. Neil V. Whittock, Louise Izatt
    Pages 183-194
  16. Johan T. den Dunnen
    Pages 195-204
  17. Mara Giordano
    Pages 205-226
  18. Cyril Mamotte, Frank Christiansen, Lyle J. Palmer
    Pages 227-254

About this book


Powerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today.


Allele DNA Ligation Microarray PCR Quantitative PCR Southern Blot Western Blotting bioinformatics gene expression genes hybridization molecular biology

Editors and affiliations

  • John M. Walker
    • 1
  • Ralph Rapley
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-288-9
  • Online ISBN 978-1-59259-870-0
  • About this book