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Medical Biomethods Handbook

  • John M. Walker
  • Ralph Rapley

Table of contents

  1. Front Matter
    Pages i-xv
  2. Ralph Rapley
    Pages 1-12
  3. Marilena Aquino de Muro
    Pages 13-23
  4. Gareth J. S. Jenkins
    Pages 25-34
  5. Bronwen Harvey, Pirkko Soundy
    Pages 35-42
  6. Pirkko Soundy, Bronwen Harvey
    Pages 43-62
  7. Jeroen H. Roelfsema, Dorien J. M. Peters
    Pages 79-86
  8. Anu Suomalainen, Ann-Christine Syvänen
    Pages 87-94
  9. Beatriz Sanchez-Vega
    Pages 95-116
  10. Simon G. Gregory
    Pages 117-133
  11. Ioannis Bossis, Antonios Voutetakis, Constantine A. Stratakis
    Pages 135-145
  12. Marian Hill
    Pages 147-153
  13. Richard Kitching, Arun Seth
    Pages 155-165
  14. Carla Osiowy
    Pages 167-181
  15. Neil V. Whittock, Louise Izatt
    Pages 183-194
  16. Johan T. den Dunnen
    Pages 195-204
  17. Mara Giordano
    Pages 205-226
  18. Cyril Mamotte, Frank Christiansen, Lyle J. Palmer
    Pages 227-254
  19. Phillip G. Febbo
    Pages 255-271
  20. Katherine Loens, D. Ursi, H. Goossens, M. Ieven
    Pages 273-291
  21. Faye A. Eggerding
    Pages 293-303
  22. Jörg Dötsch, Ellen Schoof, Wolfgang Rascher
    Pages 305-313
  23. David Sugden
    Pages 327-345
  24. Herbert Oberacher, Walther Parson
    Pages 347-373
  25. Peng-Hui Wang, Yann-Jang Chen, Chi-Hung Lin
    Pages 375-386
  26. Bernd H. A. Rehm, Frank Reinecke
    Pages 387-407
  27. Amanda D. Watters
    Pages 409-418
  28. William J. Jordan
    Pages 419-427
  29. Benny K. C. Lo
    Pages 429-446
  30. Avery A. Sandberg, John F. Stone, Zhong Chen
    Pages 447-462
  31. Rachel E. Ibbotson, Anton E. Parker
    Pages 463-469
  32. Andreas Hochhaus
    Pages 471-485
  33. Irina Gromova, Pavel Gromov, Julio E. Celis
    Pages 487-506
  34. Mark P. Richards
    Pages 507-518
  35. Margaret A. Jenkins, Sujiva Ratnaike
    Pages 519-530
  36. James L. Weaver, Maryalice Stetler-Stevenson
    Pages 531-553
  37. Patricia A. Fetsch
    Pages 555-571
  38. Patricia Severino, Sylvain Brisse
    Pages 573-581
  39. Rahul Chodhari, Eddie Chung
    Pages 593-606
  40. Guy T. N. Besley
    Pages 607-623
  41. Stella B. Somiari
    Pages 625-638
  42. Back Matter
    Pages 639-644

About this book

Introduction

Powerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today.

Keywords

Allele DNA Ligation Microarray PCR Quantitative PCR Southern Blot Western Blotting bioinformatics gene expression genes hybridization molecular biology

Editors and affiliations

  • John M. Walker
    • 1
  • Ralph Rapley
    • 1
  1. 1.University of HertfordshireHatfieldUK

Bibliographic information