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Multiple Antigen Immunostaining Procedures

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Immunocytochemical Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 115))

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Abstract

The immunohistological demonstration of more than one antigen in the same tissue section may be used for studying the topographic relationships of cell populations and for correlating cell phenotype with functional or prognostic markers or with microbial infection in situ (1). In addition, single- or double-label immunohistochemistry may also be combined with the detection of nucleic acid sequences by in situ hybridization for revealing messenger RNA and translated protein side by side. The combination of these techniques is primarily dependent on the compatibility of the antigen retrieval technique needed and the stability of the RNA and DNA sequences in question (2) (see Chapter 46).

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References

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Author information

Authors and Affiliations

  1. A. Szent-Gyorgyi University of Medicine Szaged, Hungary

    Tibor Krenacs & Laszlo Krenacs

  2. National Institutes of Health, National Cancer Institute Laboratory of Pathology, Bethesda, MD

    Mark Raffeld

Authors
  1. Tibor Krenacs
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  2. Laszlo Krenacs
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  3. Mark Raffeld
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Editor information

Editors and Affiliations

  1. Catholic University of America, Washington, DC

    Lorette C. Javois

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© 1999 Humana Press Inc., Totowa, NJ

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Krenacs, T., Krenacs, L., Raffeld, M. (1999). Multiple Antigen Immunostaining Procedures. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-213-9:223

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  • DOI: https://doi.org/10.1385/1-59259-213-9:223

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-813-4

  • Online ISBN: 978-1-59259-213-5

  • eBook Packages: Springer Protocols

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