Immunocytochemical Methods and Protocols

  • Lorette C. Javois

Part of the Methods in Molecular Biology™ book series (MIMB, volume 115)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Antibody Preparation

  3. Tissue Preparation for Light Microscopic Analyses

    1. Front Matter
      Pages 43-43
    2. Gary L. Bratthauer
      Pages 57-62
    3. Gary L. Bratthauer
      Pages 63-70
    4. Gary L. Bratthauer
      Pages 71-76
    5. Gary L. Bratthauer
      Pages 77-84
  4. Light Microscopic Detection Systems

    1. Front Matter
      Pages 95-95
    2. J. Michael Mullins
      Pages 97-105
    3. Lorette C. Javois
      Pages 107-111

About this book

Introduction

In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity.
Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.

Keywords

DNA ELISA Filtration Organelle PCR cell membrane chemistry electron microscopy hybridization membrane metabolism microscopy nucleic acid protein tissue

Editors and affiliations

  • Lorette C. Javois
    • 1
  1. 1.Catholic University of AmericaWashington

Bibliographic information

  • DOI https://doi.org/10.1385/1592592139
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-813-4
  • Online ISBN 978-1-59259-213-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book