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Immunocytochemical Methods and Protocols

  • Lorette C. Javois

Part of the Methods in Molecular Biology™ book series (MIMB, volume 115)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Antibody Preparation

  3. Tissue Preparation for Light Microscopic Analyses

    1. Front Matter
      Pages 43-43
    2. Gary L. Bratthauer
      Pages 57-62
    3. Gary L. Bratthauer
      Pages 63-70
    4. Gary L. Bratthauer
      Pages 71-76
    5. Gary L. Bratthauer
      Pages 77-84
  4. Light Microscopic Detection Systems

    1. Front Matter
      Pages 95-95
    2. J. Michael Mullins
      Pages 97-105
    3. Lorette C. Javois
      Pages 107-111
    4. Virginia M. Heatwole
      Pages 141-148
    5. Lorette C. Javois, J. Michael Mullins
      Pages 175-179
    6. Tibor Krenacs, Laszlo Krenacs, Mark Raffeld
      Pages 223-233
    7. Constance Oliver
      Pages 241-245
  5. Fluorescence Activated Cell Sorter (FACS)Analyses

    1. Front Matter
      Pages 247-247
    2. Robert E. Cunningham
      Pages 257-260
    3. Robert E. Cunningham
      Pages 261-263
    4. Robert E. Cunningham
      Pages 265-270
    5. Robert E. Cunningham
      Pages 271-273
    6. Robert E. Cunningham
      Pages 275-279
    7. Liana Harvath, Douglas A. Terle
      Pages 281-290
    8. Liana Harvath
      Pages 291-298
    9. Liana Harvath, Robert R. Aksamit, Robert E. Cunningham
      Pages 299-308
    10. Liana Harvath, Douglas A. Terle
      Pages 309-315
  6. Colloidal Gold Detection Systems for Electron Microscopic Analyses

    1. Front Matter
      Pages 317-317
    2. Constance Oliver, Maria Celia Jamur
      Pages 319-326
    3. Constance Oliver
      Pages 327-330
    4. Constance Oliver
      Pages 331-334
    5. Constance Oliver
      Pages 335-339
    6. Constance Oliver
      Pages 341-345

About this book

Introduction

In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity.
Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases.

Keywords

DNA ELISA Filtration Organelle PCR cell membrane chemistry electron microscopy hybridization membrane metabolism microscopy nucleic acid protein tissue

Editors and affiliations

  • Lorette C. Javois
    • 1
  1. 1.Catholic University of AmericaWashington

Bibliographic information

  • DOI https://doi.org/10.1385/1592592139
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-813-4
  • Online ISBN 978-1-59259-213-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site