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In vivo antidiabetic and antioxidant activities of Prosopis africana fruit extracts in streptozotocin-induced diabetic Wistar rats

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Abstract

The effect of Prosopis africana fruit extracts on biochemical indices, blood glucose level, lipid profile, and its antioxidant potentials in diabetic rats was studied. In addition, structural elucidation of the extracts with HPLC was carried out. Sixty male Wistar rats, grouped into 10 were used. Groups II–X were given a single intraperitoneal dose of 40 mg/kg freshly prepared streptozotocin injection. Groups I and II were normal and diabetic control respectively. Groups III–V and VII–IX were treated with 50, 100, and 200 mg/kg coconut water extract and aqueous extract of the fruit, respectively. Group VI was treated with 1 ml/kg coconut water only while group X was treated with 21.4 mg/kg of metformin. Treatment lasted for 14 days. Hence, the animals were sacrificed liver and kidney were excised and used for various analyses. HPLC revealed compounds like ferrulic acid, isovitexin, and vicenin in coconut water extract only. At the end of the experiment, diabetic rats administered extracts (especially coconut water extract) demonstrated significant (p < 0.05) decrease in blood glucose and bilirubin levels, as well as serum AST, ALP, ALT activities, and lipid profiles. Also, diabetic rats administered extracts exhibited significant (p < 0.05) increase in albumin and HDL levels, as well as serum antioxidant enzyme activities (p < 0.05) when compared with the diabetic control. Hence, coconut water extract may be useful in managing diabetes mellitus conditions.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria

    F. A. Bamisaye & O. A. Ayodele

  2. Redox Biology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria

    O. R. Ajuwon

  3. Department of Plant Science and Biotechnology, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria

    A. O. Oluwajobi

  4. Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria

    B. O. Ajiboye

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Contributions

All authors participated in the design, interpretation of the studies, and analysis of the data and review of the manuscript; BFA, AOA, ORA, AOO, and ABO conducted the experiments, wrote the manuscript, and approved for submission.

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Correspondence to B. O. Ajiboye.

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Bamisaye, F.A., Ayodele, O.A., Ajuwon, O.R. et al. In vivo antidiabetic and antioxidant activities of Prosopis africana fruit extracts in streptozotocin-induced diabetic Wistar rats. Nutrire 48, 29 (2023). https://doi.org/10.1186/s41110-023-00212-z

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