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Increased Expression of Kindlin 2 in Luteinized Granulosa Cells Correlates With Androgen Receptor Level in Patients With Polycystic Ovary Syndrome Having Hyperandrogenemia

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Abstract

Hyperandrogenemia is the leading defect in patients with polycystic ovary syndrome (PCOS) and considered to be involved in the ovulation dysfunction of PCOS. During the process of ovulation, granulosa cells (GCs) undergo epithelial–mesenchymal transition (EMT), and integrin-interacting protein kindlin 2 is a well-known regulator in EMT. Therefore, our objective here was to compare the expression levels of kindlin 2 in luteinized GCs between patients with PCOS and control women and the relationship between kindlin 2 and PCOS pathogenesis. In this study, kindlin 2 expression was significantly increased in luteinized GCs from patients with PCOS, and kindlin 2 could be induced by testosterone both in vitro and in vivo. Meanwhile, kindlin 2 was positively correlated with androgen receptor (AR) in PCOS GCs. Taken together, kindlin 2 may play a role in luteinized GCs, especially in the case of excess androgen. Further studies are required to assess the specific role of kindlin 2 in follicular development and PCOS pathogenesis.

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Authors and Affiliations

  1. Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, and Laboratory of Stem Cell, Development and Reproductive Medicine, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, #38 Xue Yuan Road, Beijing, 100191, China

    Mei Yang PhD, Juan Du PhD, Danyu Lu PhD, Caixia Ren PhD & Hongquan Zhang PhD

  2. Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Second Hospital, Xizhimen, Beijing, 100044, China

    Huan Shen MD, PhD & Xi Chen MD, PhD

  3. Key Laboratory of Assisted Reproduction, Ministry of Education, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China

    Jie Qiao MD, PhD

Authors
  1. Mei Yang PhD
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  2. Juan Du PhD
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  3. Danyu Lu PhD
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  4. Caixia Ren PhD
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  5. Huan Shen MD, PhD
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  6. Jie Qiao MD, PhD
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  7. Xi Chen MD, PhD
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  8. Hongquan Zhang PhD
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Corresponding authors

Correspondence to Xi Chen MD, PhD or Hongquan Zhang PhD.

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Yang, M., Du, J., Lu, D. et al. Increased Expression of Kindlin 2 in Luteinized Granulosa Cells Correlates With Androgen Receptor Level in Patients With Polycystic Ovary Syndrome Having Hyperandrogenemia. Reprod. Sci. 21, 696–703 (2014). https://doi.org/10.1177/1933719113512536

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  • DOI: https://doi.org/10.1177/1933719113512536

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