Buckling along boundaries of elastic contrast as a mechanism for early vertebrate morphogenesis

Abstract.

We have investigated the mechanism of formation of the body of a typical vertebrate, the chicken. We find that the body forms initially by folding at boundaries of stiffness contrast. These boundaries are dynamic lines, separating domains of different cell sizes, that are advected in a deterministic thin-film visco-elastic flow. While initially roughly circular, the lines of elastic contrast form large “peanut” shapes evoking a slender figure-8 at the moment of formation of the animal body, due to deformation and flow in a quadrupolar stretch caused by mesoderm migration. Folding of these “peanut” or “figure-8” motives along the lines of stiffness contrast creates the global pattern of the animal, and segregates several important territories. The main result is that the pattern of cell texture in the embryo serves simultaneously two seemingly different purposes: it regionalizes territories that will differentiate to different cell types and it also locks the folds that physically segregate these territories. This explains how the different cellular types segregate in physically separated domains.

Graphical abstract