Skip to main content
Log in

Genetic Polymorphism of Natural Isolates of Nosema pyrausta (Microsporidia: Nosematidae)

  • Published:
Russian Journal of Genetics: Applied Research

Abstract

A comparison of the small subunit rRNA gene sequences of the microsporidian isolates from corn borers of the Ostrinia genus from Russia and Belarus indicates that they belong to the Nosema pyrausta species. In order to analyze genetic polymorphism, the nucleotide sequences of the molecular clones of the rRNA intergenic spacer (IGS) of the N. pyrausta isolates from Ostrinia nubilalis and Ostrinia scapulalis (Krasnodar territory), as well as from O. nubilalis (Gomel region, Belarus), are determined. Based on the information obtained and available in Genbank, a high level of divergence of the IGS locus between the isolates of different species and populations of microsporidia, as well as between the molecular clones within individual isolates of parasites, is shown.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Karpov, S.A., Mamkaeva, M.A., Aleoshin, V.V., et al., Morphology, phylogeny, and ecology of the aphelids (Aphelidea, Opisthokonta) and proposal for the new superphylum Opisthosporidia, Front. Microbiol., 2014, vol. 28, no. 5, p. 112. doi 10.3389/fmicb.2014.00112

    Google Scholar 

  2. Issi, I.V., Microsporidia as a type of parasitic protozoa, in Mikrosporidii. Seriya “Protozoologiya” (Microsporidia. Protozoology Series), Leningrad: Nauka, 1986, vol. 10, pp. 6–136.

    Google Scholar 

  3. Becnel, J.J. and Andreadis, T.G., Microsporidia in insects, in The Microsporidia and Microsporidiosis, Washington, DC: ASM Press, 1999.

    Google Scholar 

  4. Rode, N.O., Lievens, E.J., Segard, A., et al., Cryptic microsporidian parasites differentially affect invasive and native Artemia spp., Int. J. Parasitol., 2013, vol. 43, no. 10, pp. 795–803. doi 10.1016/j.ijpara.2013.04.009

    Article  PubMed  Google Scholar 

  5. Bacela-Spychalska, K., Rigaud, T., and Wattier, R.A., A co-invasive microsporidian parasite that reduces the predatory behaviour of its host Dikerogammarus villosus (Crustacea, Amphipoda), Parasitology, 2014, vol. 141, no. 2, pp. 254–258. doi 10.1017/S0031182013001510

    Article  PubMed  CAS  Google Scholar 

  6. Gegner, T., Otti, O., Tragust, S., and Feldhaar, H., Do microsporidia function as “biological weapon” for Harmonia axyridis under natural conditions?, Insect Sci., 2015, vol. 22, no. 3, pp. 353–359. doi 10.1111/1744-7917.12224

    Article  PubMed  Google Scholar 

  7. Vilcinskas, A., Schmidtberg, H., Estoup, A., et al., Evolutionary ecology of microsporidia associated with the invasive ladybird Harmonia axyridis, Insect Sci., 2015, vol. 22, no. 3, pp. 313–324. doi 10.1111/1744-7917.12159

    Article  PubMed  Google Scholar 

  8. Fries, I., Nosema ceranae in European honey bees (Apis mellifera), J. Invertebr. Pathol., 2010, vol. 103, no. 1, pp. S73–S79. doi 10.1016/j.jip.2009.06.017

    Article  PubMed  Google Scholar 

  9. Paillot, A., Sur deux protozoaires nouveaux parasites des chenilles de Pyrausta nubilalis Hubner, CR Acad. Sci. Paris, 1927, vol. 185, pp. 673–675.

    Google Scholar 

  10. Lipa, J.J., Thelohania ostriniae n. sp., a new microsporidian parasite of the European corn borer Ostrinia nubilalis Hnb. (Lepidoptera, Pyralidae), Acta Protozool., 1977, vol. 16, no. 1, pp. 151–155.

    Google Scholar 

  11. Pezzutti, R. and Serini Bolchi, G., Ostrinia nubilalis (Hb.) (Lepidoptera Pyralidae) e il suo parassita Perezia pyraustae Paillot (Sporozoa Microsporidia) in colture maidicole lombarde, Boll. Zool. Agr. Bachicolt., 1977, vol. 14, pp. 181–188.

    Google Scholar 

  12. Cagáň, L’., Bokor, P., and Plačková, A., Dissemination of the parasite Nosema pyrausta in populations of European corn borer (Ostrinia nubilalis) in Slovakia, the Czech Republic and Poland, Sci. J. Phytotech. Zootech., 1998, vol. 9, no. 3, pp. 81–85.

    Google Scholar 

  13. Malysh, Yu.M., Tokarev, Yu.S., Sitnikova, N.V., et, al., Incidence of microsporidian infection of stem borers of the genus Ostrinia (Lepidoptera: Crambidae) in the Krasnodar krai, Parazitologiya, 2011, vol. 45, no. 3, pp. 234–244.

    Google Scholar 

  14. Caffrey, D.J. and Worthley, L.H., A progress report on the investigations of the European corn borer, USDA Agric. Bull., 1927, vol. 1476, pp. 1–155.

    Google Scholar 

  15. Hill, R.E. and Gary, W.J., Effects of the microsporidium, Nosema pyrausta, on field populations of European corn borers in Nebraska, Environ. Entomol., 1979, vol. 8, pp. 91–95.

    Google Scholar 

  16. Siegel, J.P., Maddox, J.V., and Ruesink, W.G., Seasonal progress of Nosema pyrausta in the European corn borer, Ostrinia nubilalis, J. Invertebr. Pathol., 1988, vol. 52, no. 1, pp. 130–136.

    Article  Google Scholar 

  17. Lewis, L.C., Sumerford, D.V., Bing, L.A., and Gunnarson, R.D., Dynamics of Nosema pyrausta in natural populations of the European corn borer, Ostrinia nubilalis: A six-year study, Biocontrol, 2006, vol. 51, pp. 627–642.

    Google Scholar 

  18. Lewis, L.C., Bruck, D.J., Prasifka, J.R., and Raun, E.S., Nosema pyrausta: Its biology, history, and potential role in a landscape of transgenic insecticidal crops, Biol. Control, 2009, vol. 48, pp. 223–231.

    Article  Google Scholar 

  19. Sambrook, J., Fritsch, E., and Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989.

    Google Scholar 

  20. Weiss, L.M. and Vossbrinck, C.R., Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the microsporidia, in The Microsporidia and Microsporidiosis, Washington, DC: ASM Press, 1999, pp. 129–171.

    Google Scholar 

  21. Huang, W.F., Tsai, S.J., Lo, C.F., et al., The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis, Fungal Genet. Biol., 2004, vol. 41, no. 5, pp. 473–481.

    Article  PubMed  CAS  Google Scholar 

  22. Vogelstein, B. and Gillespie, D., Preparative and analytical purification of DNA from agarose, Proc. Natl. Acad. Sci. U.S.A., 1979, vol. 76, pp. 615–619.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  23. Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symp., 1999, vol. 41, pp. 95–98.

    CAS  Google Scholar 

  24. Liu, H., Pan, G., Luo, B., et al., Intraspecific polymorphism of rDNA among five Nosema bombycis isolates from different geographic regions in China, J. Invertebr. Pathol., 2013, vol. 113, no. 1, pp. 63–69. doi 10.1016/j.jip.2013.01.008

    Article  PubMed  CAS  Google Scholar 

  25. Fu, Z., He, X., Cai, S., et al., Quantitative PCR for detection of Nosema bombycis in single silkworm eggs and newly hatched larvae, J. Microbiol. Methods, 2016, vol. 120, pp. 72–78. doi 10.1016/j.mimet.2015.12.003

    Article  PubMed  CAS  Google Scholar 

  26. Tokarev, Yu.S., Malysh, J.M., Kononchuk, A.G., et al., Redefinition of Nosema pyrausta (Perezia pyraustae Paillot) basing upon ultrastructural and molecular phylogenetic studies, Parasitol. Res., 2015, vol. 114, no. 2, pp. 759–761. doi 10.1007/s00436-014-4272-3

    Article  PubMed  Google Scholar 

  27. Tokarev, Yu.S., Voronin, V.N., Senderskii, I.V., and Issi, I.V., The microsporidium Glugea gasterostei Voronin 1974 (Microsporidia: Marinosporidia) from the three-spined stickleback Gasterosteus aculeatus (Actinopterygii: Gasterosteiformes) as an independent species, Parazitologiya, 2015, vol. 49, pp. 81–92.

    CAS  Google Scholar 

  28. Franzen, C., Fischer, S., Schroeder, J., et al., Morphological and molecular investigations of Tubulinosema ratisbonensis gen. nov., sp. nov. (Microsporidia: Tubulinosematidae fam. nov.), a parasite infecting a laboratory colony of Drosophila melanogaster (Diptera: Drosophilidae), J. Eukaryot. Microbiol., 2015, vol. 52, pp. 141–52.

    Article  Google Scholar 

  29. Bjornson, S., Le, J., Saito, T., and Wang, H., Ultrastructure and molecular characterization of a microsporidium, Tubulinosema hippodamiae, from the convergent lady beetle, Hippodamia convergens Guerin-Meneville, J. Invertebr. Pathol., 2011, vol. 206, pp. 280–288.

    Google Scholar 

  30. Malysh, J.M., Tokarev, Y.S., Sitnicova, N.V., et al., Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia, Acta Protozool., 2013, vol. 52, pp. 299–308.

    Google Scholar 

  31. Trepashko, L.I., Nadtochaeva, S.V., and Golovach, V.V., Dangerous pests of maize, Zashch. Karantin Rast., 2012, no. 9, pp. 44–49.

    Google Scholar 

  32. Ironside, J.E., Diversity and recombination of dispersed ribosomal DNA and protein coding genes in Microsporidia, PLoS ONE, 2013, vol. 8, no. 2. doi 10.1371/journal.pone.0055878

    Google Scholar 

  33. Sagastume, S., del Aguila, C., Martin-Hernandez, R., et al., Polymorphism and recombination for rDNA in the putatively asexual microsporidian Nosema ceranae, a pathogen of honeybees, Environ. Microbiol., 2011, vol. 13, no. 1, pp. 84–95. doi 10.1111/j.1462-2920.2010.02311.x

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to I. V. Grushevaya.

Additional information

Original Russian Text © I.V. Grushevaya, A.N. Ignatieva, J.M. Malysh, L.I. Trepashko, Yu.S. Tokarev, A.N. Frolov, 2017, published in Ecologicheskaya Genetika, 2017, Vol. 15, No. 1, pp. 38–43.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Grushevaya, I.V., Ignatieva, A.N., Malysh, J.M. et al. Genetic Polymorphism of Natural Isolates of Nosema pyrausta (Microsporidia: Nosematidae). Russ J Genet Appl Res 8, 135–139 (2018). https://doi.org/10.1134/S2079059718020053

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079059718020053

Keywords

Navigation