Abstract
A comparison of the small subunit rRNA gene sequences of the microsporidian isolates from corn borers of the Ostrinia genus from Russia and Belarus indicates that they belong to the Nosema pyrausta species. In order to analyze genetic polymorphism, the nucleotide sequences of the molecular clones of the rRNA intergenic spacer (IGS) of the N. pyrausta isolates from Ostrinia nubilalis and Ostrinia scapulalis (Krasnodar territory), as well as from O. nubilalis (Gomel region, Belarus), are determined. Based on the information obtained and available in Genbank, a high level of divergence of the IGS locus between the isolates of different species and populations of microsporidia, as well as between the molecular clones within individual isolates of parasites, is shown.
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Karpov, S.A., Mamkaeva, M.A., Aleoshin, V.V., et al., Morphology, phylogeny, and ecology of the aphelids (Aphelidea, Opisthokonta) and proposal for the new superphylum Opisthosporidia, Front. Microbiol., 2014, vol. 28, no. 5, p. 112. doi 10.3389/fmicb.2014.00112
Issi, I.V., Microsporidia as a type of parasitic protozoa, in Mikrosporidii. Seriya “Protozoologiya” (Microsporidia. Protozoology Series), Leningrad: Nauka, 1986, vol. 10, pp. 6–136.
Becnel, J.J. and Andreadis, T.G., Microsporidia in insects, in The Microsporidia and Microsporidiosis, Washington, DC: ASM Press, 1999.
Rode, N.O., Lievens, E.J., Segard, A., et al., Cryptic microsporidian parasites differentially affect invasive and native Artemia spp., Int. J. Parasitol., 2013, vol. 43, no. 10, pp. 795–803. doi 10.1016/j.ijpara.2013.04.009
Bacela-Spychalska, K., Rigaud, T., and Wattier, R.A., A co-invasive microsporidian parasite that reduces the predatory behaviour of its host Dikerogammarus villosus (Crustacea, Amphipoda), Parasitology, 2014, vol. 141, no. 2, pp. 254–258. doi 10.1017/S0031182013001510
Gegner, T., Otti, O., Tragust, S., and Feldhaar, H., Do microsporidia function as “biological weapon” for Harmonia axyridis under natural conditions?, Insect Sci., 2015, vol. 22, no. 3, pp. 353–359. doi 10.1111/1744-7917.12224
Vilcinskas, A., Schmidtberg, H., Estoup, A., et al., Evolutionary ecology of microsporidia associated with the invasive ladybird Harmonia axyridis, Insect Sci., 2015, vol. 22, no. 3, pp. 313–324. doi 10.1111/1744-7917.12159
Fries, I., Nosema ceranae in European honey bees (Apis mellifera), J. Invertebr. Pathol., 2010, vol. 103, no. 1, pp. S73–S79. doi 10.1016/j.jip.2009.06.017
Paillot, A., Sur deux protozoaires nouveaux parasites des chenilles de Pyrausta nubilalis Hubner, CR Acad. Sci. Paris, 1927, vol. 185, pp. 673–675.
Lipa, J.J., Thelohania ostriniae n. sp., a new microsporidian parasite of the European corn borer Ostrinia nubilalis Hnb. (Lepidoptera, Pyralidae), Acta Protozool., 1977, vol. 16, no. 1, pp. 151–155.
Pezzutti, R. and Serini Bolchi, G., Ostrinia nubilalis (Hb.) (Lepidoptera Pyralidae) e il suo parassita Perezia pyraustae Paillot (Sporozoa Microsporidia) in colture maidicole lombarde, Boll. Zool. Agr. Bachicolt., 1977, vol. 14, pp. 181–188.
Cagáň, L’., Bokor, P., and Plačková, A., Dissemination of the parasite Nosema pyrausta in populations of European corn borer (Ostrinia nubilalis) in Slovakia, the Czech Republic and Poland, Sci. J. Phytotech. Zootech., 1998, vol. 9, no. 3, pp. 81–85.
Malysh, Yu.M., Tokarev, Yu.S., Sitnikova, N.V., et, al., Incidence of microsporidian infection of stem borers of the genus Ostrinia (Lepidoptera: Crambidae) in the Krasnodar krai, Parazitologiya, 2011, vol. 45, no. 3, pp. 234–244.
Caffrey, D.J. and Worthley, L.H., A progress report on the investigations of the European corn borer, USDA Agric. Bull., 1927, vol. 1476, pp. 1–155.
Hill, R.E. and Gary, W.J., Effects of the microsporidium, Nosema pyrausta, on field populations of European corn borers in Nebraska, Environ. Entomol., 1979, vol. 8, pp. 91–95.
Siegel, J.P., Maddox, J.V., and Ruesink, W.G., Seasonal progress of Nosema pyrausta in the European corn borer, Ostrinia nubilalis, J. Invertebr. Pathol., 1988, vol. 52, no. 1, pp. 130–136.
Lewis, L.C., Sumerford, D.V., Bing, L.A., and Gunnarson, R.D., Dynamics of Nosema pyrausta in natural populations of the European corn borer, Ostrinia nubilalis: A six-year study, Biocontrol, 2006, vol. 51, pp. 627–642.
Lewis, L.C., Bruck, D.J., Prasifka, J.R., and Raun, E.S., Nosema pyrausta: Its biology, history, and potential role in a landscape of transgenic insecticidal crops, Biol. Control, 2009, vol. 48, pp. 223–231.
Sambrook, J., Fritsch, E., and Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989.
Weiss, L.M. and Vossbrinck, C.R., Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the microsporidia, in The Microsporidia and Microsporidiosis, Washington, DC: ASM Press, 1999, pp. 129–171.
Huang, W.F., Tsai, S.J., Lo, C.F., et al., The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis, Fungal Genet. Biol., 2004, vol. 41, no. 5, pp. 473–481.
Vogelstein, B. and Gillespie, D., Preparative and analytical purification of DNA from agarose, Proc. Natl. Acad. Sci. U.S.A., 1979, vol. 76, pp. 615–619.
Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symp., 1999, vol. 41, pp. 95–98.
Liu, H., Pan, G., Luo, B., et al., Intraspecific polymorphism of rDNA among five Nosema bombycis isolates from different geographic regions in China, J. Invertebr. Pathol., 2013, vol. 113, no. 1, pp. 63–69. doi 10.1016/j.jip.2013.01.008
Fu, Z., He, X., Cai, S., et al., Quantitative PCR for detection of Nosema bombycis in single silkworm eggs and newly hatched larvae, J. Microbiol. Methods, 2016, vol. 120, pp. 72–78. doi 10.1016/j.mimet.2015.12.003
Tokarev, Yu.S., Malysh, J.M., Kononchuk, A.G., et al., Redefinition of Nosema pyrausta (Perezia pyraustae Paillot) basing upon ultrastructural and molecular phylogenetic studies, Parasitol. Res., 2015, vol. 114, no. 2, pp. 759–761. doi 10.1007/s00436-014-4272-3
Tokarev, Yu.S., Voronin, V.N., Senderskii, I.V., and Issi, I.V., The microsporidium Glugea gasterostei Voronin 1974 (Microsporidia: Marinosporidia) from the three-spined stickleback Gasterosteus aculeatus (Actinopterygii: Gasterosteiformes) as an independent species, Parazitologiya, 2015, vol. 49, pp. 81–92.
Franzen, C., Fischer, S., Schroeder, J., et al., Morphological and molecular investigations of Tubulinosema ratisbonensis gen. nov., sp. nov. (Microsporidia: Tubulinosematidae fam. nov.), a parasite infecting a laboratory colony of Drosophila melanogaster (Diptera: Drosophilidae), J. Eukaryot. Microbiol., 2015, vol. 52, pp. 141–52.
Bjornson, S., Le, J., Saito, T., and Wang, H., Ultrastructure and molecular characterization of a microsporidium, Tubulinosema hippodamiae, from the convergent lady beetle, Hippodamia convergens Guerin-Meneville, J. Invertebr. Pathol., 2011, vol. 206, pp. 280–288.
Malysh, J.M., Tokarev, Y.S., Sitnicova, N.V., et al., Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia, Acta Protozool., 2013, vol. 52, pp. 299–308.
Trepashko, L.I., Nadtochaeva, S.V., and Golovach, V.V., Dangerous pests of maize, Zashch. Karantin Rast., 2012, no. 9, pp. 44–49.
Ironside, J.E., Diversity and recombination of dispersed ribosomal DNA and protein coding genes in Microsporidia, PLoS ONE, 2013, vol. 8, no. 2. doi 10.1371/journal.pone.0055878
Sagastume, S., del Aguila, C., Martin-Hernandez, R., et al., Polymorphism and recombination for rDNA in the putatively asexual microsporidian Nosema ceranae, a pathogen of honeybees, Environ. Microbiol., 2011, vol. 13, no. 1, pp. 84–95. doi 10.1111/j.1462-2920.2010.02311.x
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Original Russian Text © I.V. Grushevaya, A.N. Ignatieva, J.M. Malysh, L.I. Trepashko, Yu.S. Tokarev, A.N. Frolov, 2017, published in Ecologicheskaya Genetika, 2017, Vol. 15, No. 1, pp. 38–43.
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Grushevaya, I.V., Ignatieva, A.N., Malysh, J.M. et al. Genetic Polymorphism of Natural Isolates of Nosema pyrausta (Microsporidia: Nosematidae). Russ J Genet Appl Res 8, 135–139 (2018). https://doi.org/10.1134/S2079059718020053
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DOI: https://doi.org/10.1134/S2079059718020053