Skip to main content
SpringerLink
Log in

The Effect of high hydrostatic pressure on the viability and mutagenesis of Salmonella typhimurium

  • Published:
Russian Journal of Genetics: Applied Research

Abstract

This work is devoted to the study of the influence of high hydrostatic pressure (HHP) on the viability and level of mutagenesis of Salmonella typhimurium. It was established that the viability of bacteria significantly decreases under hydrostatic pressure of 200 MPa or higher. In addition, the viability index of the bacteria is six orders of magnitude lower with respect to the number of colony-forming units (CFUs) compared to the data of the flow cytofluorometry analysis. This is probably due to the transition of some part of the bacterial population to a viable but nonculturable state (VBNC). HHP of 50 MPa caused a 1.9-fold increase in the number of His+ revertants of the S. typhimurium strain TA98, which indicates the potential of the induction of gene mutations under these conditions. The mechanisms to reduce the viability and genetic changes in bacterial cells under HHP conditions are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aertsen, A., De Spiegeleer, P., Vanoirbeek, K., et al., Induction of oxidative stress by high hydrostatic pressure in Escherichia coli, Appl. Environ. Microbiol., 2005, vol. 71, no. 5, pp. 2226–2231.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Aertsen, A., Houdt, R., Vanoirbeek, K., et al., An SOS response induced by high pressure in Escherichia coli, J. Bacteriol., 2004, vol. 186, no. 18, pp. 6133–6141.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Aertsen, A., Meersman, F., Hendrickx, M.E., et al., Biotechnology under high pressure: Applications and implications, Trends Biotechnol., 2009, vol. 27, no. 7, pp. 434–441.

    Article  CAS  PubMed  Google Scholar 

  • Alpas, H., Kalchayanand, N., Bozoglu, F., et al., Variation in resistance to hydrostatic pressure among strains of foodborne pathogens, Appl. Environ. Microbiol., 1999, vol. 65, pp. 4248–4251.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Colwell, R.R., Bacterial death revisited, in Nonculturable Microorganisms in the Environment, Grimes, D.J., Eds., Washington, D. C.: ASM Press, 2000, pp. 325–342.

    Chapter  Google Scholar 

  • Fernandes, P.M., Domitrovic, T., Kao, C.M., et al., Genomic expression pattern in Saccharomyces cerevisiae cells in response to high hydrostatic pressure, FEBS Lett., 2004, vol. 556, nos. 1–3, pp. 153–160.

    Article  CAS  PubMed  Google Scholar 

  • Gao, X., Li, J., and Ruan, K.C., Barotolerant Escherichia coli induced by high hydrostatic pressure, Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai), 2001, vol. 33, p. 77–81.

    CAS  PubMed  Google Scholar 

  • Golod, N.A., Loiko, N.G., Mulyukin, A.L., et al., Adaptation of lactic acid bacteria to adverse growth conditions, Mikrobiologiya, 2009, vol. 78, no. 3, pp. 317–335.

    CAS  Google Scholar 

  • Gross, J.A., Pressure-induced color mutation of Euglena gracilis, Science, 1965, vol. 147, no. 3659, pp. 741–742.

    Article  CAS  PubMed  Google Scholar 

  • Hauben, K.J., Bartlett, D.H., Soontjens, C.C., et al., Escherichia coli mutants resistant to inactivation by high hydrostatic pressure, Appl. Environ. Microbiol., 1997, vol. 63, no. 3, pp. 945–950.

    CAS  PubMed  PubMed Central  Google Scholar 

  • High-Pressure Microbiology, Michiels, Ch., Bartlett, D.H., and Aertsen, A., Eds., Washington: ASM Press, 2008.

  • Holzapfel, W.B. and Isaacs, N.S., High-Pressure Techniques in Chemistry and Physics. A Practical Approach, Oxford, New York, Tokyo: Oxford University Press, 1997.

    Google Scholar 

  • Jannasch, H.W. and Taylor, C.D., Deep-sea microbiology, Annu. Rev. Microbiol., 1984, vol. 38, pp. 487–514.

    Article  CAS  PubMed  Google Scholar 

  • Karatzas, K.A. and Bennik, M.H., Characterization of a Listeria monocytogenes Scott A isolate with high tolerance towards high hydrostatic pressure, Appl. Environ. Microbiol., 2002, vol. 68, pp. 3183–3189.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kato, C., Li, L., Nogi, Y., et al., Extremely barophilic bacteria isolated from the Mariana Trench, Challenger Deep, at a depth of 11000 meters, Appl. Environ. Microbiol., 1998, vol. 64, no. 4, pp. 1510–1513.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kryazhevskih, N.A., Demkina, E.V., Manucharova, N.A., et al., Reactivation of resting and nonculturable forms of bacteria from ancient soils and frozen subsoil deposits, Mikrobiologiya, 2012, vol. 81, no. 4, pp. 474–485.

    Google Scholar 

  • Meersman, F. and McMillan, P.F., High hydrostatic pressure: A probing tool and a necessary parameter in biophysical chemistry, Chem. Commun., 2014, vol. 50, pp. 766–775.

    Article  CAS  Google Scholar 

  • Mortelmans, K. and Zeiger, E., The Ames Salmonella/microsome mutagenicity assay, Mutat. Res., 2000, vol. 455, nos. 1–2, pp. 29–60.

    Article  CAS  PubMed  Google Scholar 

  • Mota, M.J., Lopes, R.L., Delgadillo, I., et al., Microorganisms under high pressure—adaptation, growth and biotechnological potential, Biotechnol. Adv., 2013, vol. 31, no. 8, pp. 1426–1434.

    Article  CAS  PubMed  Google Scholar 

  • Nogi, Y., Kato, C., and Horikoshi, K., Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov., Arch. Microbiol., 1998, vol. 170, no. 5, pp. 331–338.

    Article  CAS  PubMed  Google Scholar 

  • Oger, Ph.J. and Jebbar, M., The many ways of coping with pressure, Res. Microbiol., 2010, vol. 161, no. 10, pp. 799–809.

    Article  PubMed  Google Scholar 

  • Radman, M., Fidelity and infidelity, Nature, 2001, vol. 413, no. 15, pp. 413–115.

    Google Scholar 

  • Ritz, M., Tholozan, J.L., Fedeeighi, M., et al., Morphological and physiological characterization of Listeria monocytogenes subjected to high hydrostatic pressure, Appl. Environ. Microbiol., 2001, vol. 67, no. 5, pp. 2240–2247.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rosin, M.P. and Zimmerman, A.M., The induction of cytoplasmic petite mutants of Saccharomyces cerevisiae by hydrostatic pressure, J. Cell Sci., 1977, vol. 26, pp. 373–385.

    CAS  PubMed  Google Scholar 

  • Vanlint, D., Mitchell, R., Bailey, E., et al., Rapid acquisition of gigapascal-high-pressure resistance by Escherichia coli, mBio, 2011, vol. 2, no. 1, pp. 130–110.

    Article  Google Scholar 

  • Wachtershauser, G., From volcanic origins of chemoautotrophic life to Bacteria, Archaea and Eukarya, Phil. Tran. R. Soc. B, 2006, vol. 361, pp. 1787–1808.

    Article  Google Scholar 

  • Winter, R. and Jeworrek, C., Effect of pressure on membranes, Soft Matter, 2009, vol. 5, no. 17, pp. 3157–3173.

    Article  CAS  Google Scholar 

  • Yudin, I.P., Modern approaches to bacteria viability assessment with an emphasis on phenomenon of the viable but non-culturable state, Ann. Mechnikov Inst., 2007, vol. 3, pp. 8–16.

    Google Scholar 

  • Zeng, X., Birrien, J.L., Fouquet, Y., et al., Pyrococcus CH1, an obligate piezophilic hyperthermophile: Extending the upper pressure-temperature limits for life, ISME J., 2009, vol. 3, no. 7, pp. 873–876.

    Article  CAS  PubMed  Google Scholar 

  • Zhuk, A.S., Experimental model for evaluating effects of environmental stressors, Master’s Dissertation, Saint Petersburg, 2010.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kazan (Volga region) Federal University, Kazan, 420008, Russia

    N. S. Karamova, P. V. Zelenikhin, V. D. Kiselev, A. A. Lipatnikova & O. N. Ilinskaya

Authors
  1. N. S. Karamova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. V. Zelenikhin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. D. Kiselev
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Lipatnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. O. N. Ilinskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. S. Karamova.

Additional information

Original Russian Text © N.S. Karamova, P.V. Zelenikhin, V.D. Kiselev, A.A. Lipatnikova, O.N. Ilinskaya, 2015, published in Ecologicheskaya Genetika, 2015, Vol. 13, No. 4, pp. 99–107.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Karamova, N.S., Zelenikhin, P.V., Kiselev, V.D. et al. The Effect of high hydrostatic pressure on the viability and mutagenesis of Salmonella typhimurium . Russ J Genet Appl Res 7, 698–704 (2017). https://doi.org/10.1134/S2079059717060077

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2079059717060077

Keywords

Search

Navigation