Abstract
The paper reports on the disruptive selection performed on two-spotted spider mite females in 20 inbred generations for resistance or sensitivity to four insectoacaricides belonging to different chemical groups: dimethoate, bifenthrin, abamectin, and bromopropylate. Due to the haplodiploid reproduction mode in mites, on average about 30% of nonresistant females were present in each generation of genotype differentiation in all acaricide-resistant lines. In single females from the lines under comparison, the electrophoretic separation of the enzymes revealed the presence of carboxylesterase fractions. Mites were tested with diagnostic concentrations of dimethoate, bifenthrin, and piridaben. The synergistic effects of the monooxygenase inhibitor piperonyl butoxide on mites were compared. It was concluded that the induction of carboxylesterase and monooxygenase activities is a universal adaptive response of the arthropod organism that underlies resistance to all acutely toxic insectoacaricides.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bass, Ch. and Field, L.M., Gene amplification and insecticide resistance, Pest Manage. Sci., 2011, vol. 67, no. 8, pp. 886–890.
Belenkiy, M.L., Elementy kolichestvennoi otsenki farmacologicheskogo effekta (Elements of Quantitative Estimation of Pharmacological Action), Riga: AN Latv. SSR, 1959.
Dermauw, W., Ilias, A., Riga, M., et al., The cysloop ligand-gated ion channel gene family of Tetranychus urticae: Implications for acaricide toxicology and novel mutation associated with abamectin resistance, Insect Biochem. Mol. Biol., 2012, vol. 42, pp. 455–465.
Karnovsky, M.J. and Roots, L., A “direct-coloring” thiocholine method for cholinesterases, Histochem. Cytochem, 1964, vol. 12, pp. 219–221.
Kwon, D.H., Yoon, K.S., Clark, J.M., and Lee, S.H., A point mutation in a glutamate-gated chloride channel confers abamectin resistance in the two-spotted spider mite, Tetranychus urticae Koch, Insect Mol. Biol., 2010, vol. 19, pp. 583–591.
Leeuwen, T. and Tirry, L., Esterase-mediated bifenthrin resistance in a multiresistant strain of the two-spotted spider mite, Pest Manage. Sci., 2007, vol. 63, no. 2, pp. 150–156.
Leeuwen, T. van, Pottelberge, S., and Tirry, L., Comparative acaricide susceptibility and detoxifying enzyme activities in field-collected resistant and susceptible strains of Tetranychus urticae, Pest Manage. Sci., 2005, vol. 61, no. 5, pp. 499–507.
Leeuwen, T. van, Pottelberge S., and Tirry L., Biochemical analysis of chlorfenapyr-selected resistant strain of Tetranychus urticae Koch, Pest Manage. Sci., 2006, vol. 62, no. 5, pp. 425–433.
Maurer, H.R., Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (Working Methods in Modern Science), De Gruyter, 1971, 2nd ed.
Nyoni, B.N., Gorman, K., Mzilahowa, T., et al., Pyrethroid resistance in the tomato spider mite, Tetranychus evansi, is associated with mutation of the para-type sodium channel, Pest Manage. Sci., 2011, vol. 67, no. 8, pp. 891–897.
Singer, M. and Berg, P., Genes and Genomes: A Changing Perspective, University Science Books, 1991.
Sundukov, O.V., Etiologiya ostroi toksichnosti insektoakaritsidov i fisiologicheskie factory, opredelyayushchie izbiratel’nost’ ikh deistviya na chlenistonogikh (Aetiology of Acute Toxic Action of Insectoacaricides and Physiological Factors that Determine Selectivity of Their Action on Arthropods), St. Petersburg: Nauka, 2012.
Sundukov, O.V., Tulaeva, I.A., and Zubanov, Ye.A., Inheritance of resistance to acaricides in inbred lines of common spider mites, Ekol. Genet., 2014, vol. 12, no. 3, pp. 43–51.
Urbakh, V.Yu., Biometricheskie metody (Biometrical Methods), Moscow: Nauka, 1964.
Ya-ning, F., Shu, Zh., Wei, S., et al., The sodium channel gene in Tetranychus cinnabarinus (Boisduval): Identification and expression analysis of a mutation associated with pyrethroid resistance, Pest Manage. Sci., 2011, vol. 67, no. 8, pp. 904–912.
Zhao, X. and Salgado, V.L., The role of GABA and glutamate receptors in susceptibility and resistance to chloride channel blocker insecticides, Pestic. Biochem. Physiol., 2010, vol. 97, pp. 153–160.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.V. Sundukov, I.A. Tulaeva, E.A. Zubanov, 2015, published in Ecologicheskaya Genetika, 2015, Vol. 13, No. 3, pp. 76–84.
Rights and permissions
About this article
Cite this article
Sundukov, O.V., Tulaeva, I.A. & Zubanov, E.A. Manifestation of resistance to insectoacaricides in inbred lines of the two-spotted spider mite under disruptive selection. Russ J Genet Appl Res 7, 180–188 (2017). https://doi.org/10.1134/S2079059717020125
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079059717020125