Abstract
Modern trends in the use of DNA in nanotechnology and biotechnology are aiming to develop new methods of analysis of DNA molecules based on the developing instrument base. We have developed a method of mild nondestructive ablation to transfer DNA molecules into an aerosol phase using terahertz radiation. In this paper, the size of DNA nanoparticles in the gas phase was measured using the diffusion aerosol spectrometer.?The changes taking place with DNA in the gas phase were visualized using the atomic force microscopy (AFM). A comparison of the measurements of the diffusion sizes of the plasmid pUC18 aerosol particles with measurements using AFM gives grounds to assume that the process of condensation of the DNA molecules takes place in the gas phase. A model was constructed according to modern concepts of the process of DNA condensation and formation of globules. The theoretical calculations are in good agreement with the experimental results. The experimentally estimated persistence length of the DNA in the gas phase was about 0.5 nm, which indicates the absence of a distributed charge on the surface of the DNA in the gas phase and the nonionizing nature of terahertz radiation. The study of DNA conformation in the gas phase will expand knowledge of DNA compaction in natural and artificial conditions.
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Original Russian Text © T.N. Goryachkovskaya, A.S. Kozlov, V.M. Popik, N.A. Kolchanov, S.E. Peltek, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 4/2, pp. 1013–1021.
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Goryachkovskaya, T.N., Kozlov, A.S., Popik, V.M. et al. Dependence of a DNA globule size in a gas phase on the chain length. Russ J Genet Appl Res 5, 394–400 (2015). https://doi.org/10.1134/S2079059715040061
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DOI: https://doi.org/10.1134/S2079059715040061