Abstract
The goal of our work was to examine the localization and expression level of sirtuin 1 (SIRT1) in human dermal fibroblasts at different stages of ontogenesis, as well as to identify the role of SIRT1 in skin aging. The study was based on an autopsy skin material sampled from fetuses dying prenatally in the 20th to 40th week of gestation and from people dying in the period starting from birth to the age of 85 years. SIRT1 and the proliferating cell nuclear antigen (PCNA) were detected by the indirect immunohistochemical method. The results of our study have shown that the SIRT1 level decreases in dermal fibroblasts during the period from birth until the age of 85 and that the proliferative activity of dermal fibroblasts and their counts are steadily reduced. The decreasing content of SIRT1 in dermal fibroblasts with age correlates with a reduction of their proliferative activity and can be considered as one of the mechanisms for inhibition of the proliferation of dermal fibroblasts.
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References
Braidy, N., Poljak, A., Grant, R., et al., Differential expression of sirtuins in the aging rat brain, Front. Cell. Neurosci., 2015, vol. 9, p. 167.
Ghosh, S. and Zhou, Z., SIRTain regulators of premature senescence and accelerated aging, Protein Cell, 2015, vol. 6, no. 5, pp. 322–333.
Gunin, A.G., Kapitova, I.N., and Suslonova, N.V., Effects of histone deacetylase inhibitors on estradiolinduced proliferation and hyperplasia formation in the mouse uterus, J. Endocrinol., 2005, vol. 185, pp. 539–549.
Gunin, A.G., Petrov, V.V., Golubtzova, N.N., et al., Age-related changes in angiogenesis in human dermis, Exp. Gerontol., 2014, vol. 55, pp. 143–151.
Hwang, J.S., Ham, S.A., Yoo, T., et al., Sirtuin 1 mediates the actions of peroxisome proliferator-activated receptor d on the oxidized low-density lipoprotein-triggered migration and proliferation of vascular smooth muscle cells, Mol. Pharmacol., 2016, vol. 90, no. 5, pp. 522–529.
Kuroyanagi, G., Otsuka, T., Yamamoto, N., et al., Resveratrol suppreßses TGF-ß-induced VEGF synthesis in osteoblasts: inhibition of the p44/p42 MAPKs and SAPK/JNK pathways, Exp. Ther. Med., 2015, vol. 9, no. 6, pp. 2303–2310.
Lee, J.S., Park, K.Y., Min, H.G., et al., Negative regulation of stress-induced matrix metalloproteinase-9 by Sirt1 in skin tissue, Exp. Dermatol., 2010, vol. 19, no. 12, pp. 1060–1066.
Li, L., Sun, Q., Li, Y., et al., Overexpression of SIRT1 induced by resveratrol and inhibitor of miR-204 suppresses activation and proliferation of microglia, J. Mol. Neurosci., 2015, vol. 56, no. 4, pp. 858–867.
Li, W., Du, D., Wang, H., et al., Silent information regulator 1 (SIRT1) promotes the migration and proliferation of endothelial progenitor cells through the PI3K/Akt/eNOS signaling pathway, Int. J. Clin. Exp. Pathol., 2015, vol. 8, no. 3, pp. 2274–2287.
Liu, B., Ghosh, S., Yang, X., et al., Resveratrol rescues SIRT1-dependent adult stem cell decline and alleviates progeroid features in laminopathy-based progeria, Cell. Metab., 2012, vol. 16, no. 6, pp. 738–750.
Ma, C.Y., Yao, M.J., Zhai, Q.W., et al., SIRT1 suppresses self-renewal of adult hippocampal neural stem cells, Development, 2014, vol. 141, no. 24, pp. 4697–4709.
Mercken, E.M., Mitchell, S.J., Martin-Montalvo, A., et al., SRT2104 extends survival of male mice on a standard diet and preserves bone and muscle mass, Aging Cell, 2014, vol. 13, no. 5, pp. 787–796.
Ohguchi, K., Itoh, T., Akao, Y., et al., SIRT1 modulates expression of matrix metalloproteinases in human dermal fibroblasts, Br. J. Dermatol., 2010, vol. 163, no. 4, pp. 689–694.
Ponnusamy, M., Zhuang, M.A., Zhou, X., et al., Activation of sirtuin-1 promotes renal fibroblast activation and aggravates renal fibrogenesis, J. Pharmacol. Exp. Ther., 2015, vol. 354, no. 2, pp. 142–151.
Rathbone, C.R., Booth, F.W., and Lees, S.J., SIRT1 increases skeletal muscle precursor cell proliferation, Eur. J. Cell. Biol., 2009, vol. 88, no. 1, pp. 35–44.
Rimmele, P., Bigarella, C.L., Liang, R., et al., Aginglike phenotype and defective lineage specification in SIRT1-deleted hematopoietic stem and progenitor cells, Stem Cell Rep., 2014, vol. 3, no. 1, pp. 44–59.
Ulakcsai, Z., Bagamery, F., Vincze, I., et al., Protective effect of resveratrol against caspase 3 activation in primary mouse fibroblasts, Croat. Med. J., 2015, vol. 56, no. 2, pp. 78–84.
Yang, Q., Wang, B., Zang, W., et al., Resveratrol inhibits the growth of gastric cancer by inducing G1 phase arrest and senescence in a Sirt1-dependent manner, PLoS One, 2013, vol. 8, no. 11, p. e70627.
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Golubtsova, N.N., Filippov, F.N. & Gunin, A.G. Age-Related Changes in the Content of Sirtuin 1 in Human Dermal Fibroblasts. Adv Gerontol 7, 302–306 (2017). https://doi.org/10.1134/S207905701704004X
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DOI: https://doi.org/10.1134/S207905701704004X