Abstract
The effect the micromycete enzymatic complex has on the lignin component in tree species barks is studied using the example of Trichoderma fungi. The MG-97/6 Trichoderma asperellum Samuels VKPM F-765 strain is used as the biological object of study. The substrates are Siberian larch bark, European white birch bast, and preparations of dioxane lignin isolated from larch bark and birch bast according to Pepper’s method, i.e., via treatment with a dioxane-water mixture (9: 1) in the presence of HCl (0.7%). Trichoderma fungi are cultivated in Petri dishes placed in a thermostat for 10 days under conditions of 25–27°C, 80-% humidity, and pH 6.5. The substrate is inoculated with a mycelial suspension of the fungi in the amount of 1 × 106 spores per 1 g of the substrate (oven-dry weight). Chemical analysis, UV spectroscopy, and IR spectroscopy are used to show that the micromycete enzyme complex induces destructive changes in lignin, both in situ and in vitro. A scheme for the oxidative degradation of wood substrate lignins by the T. asperellum MG-97/6 fungi is proposed. Microbial decomposition of lignin can be recommended for the preparation of such Trichodermin biological products, using lignin-containing wastes as substrates. It can also be recommended for the destruction and bleaching of wood fibers for purposes of their further industrial use (e.g., in the production of paper and bioethanol).
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Original Russian Text © T.V. Ryazanova, N.A. Chuprova, T.A. Luneva, 2014, published in Kataliz v Promyshlennosti.
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Ryazanova, T.V., Chuprova, N.A. & Luneva, T.A. Effect of Trichoderma fungi on lignin from tree species barks. Catal. Ind. 7, 82–89 (2015). https://doi.org/10.1134/S2070050415010134
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DOI: https://doi.org/10.1134/S2070050415010134