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Identification of the Side Population Associated with ATP-Binding Cassette Transporters Activity Using Imaging Flow Cytometry

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Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract—

DyeCycle Violet efflux, caused by ATP-binding cassette transporters activity, was analyzed in human colorectal adenocarcinoma cell lines SW480, HT-29, Caco-2 by means of a FACSAria III flow cytometer and an ImageStreamX Mk II imaging flow cytometer. Besides similarity of cytometry data obtained by two instruments, the use of imaging flow cytometry made it possible to characterize the morphology of side population cells, as well as morphology of other cell populations differing in the degree of dye accumulation. The population of cells, which are smaller than the side population cells and practically do not take the dye, is of the special interest. Probably, this population may contribute to the tumor resistance to chemotherapy.

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ACKNOWLEDGMENTS

Flow cytometry and imaging flow cytometry were performed using the equipment of the Human Proteome Center for Collective Use, supported by the Ministry of Education and Science of the Russian Federation (agreement 14.621.21.0017, unique project identifier RFMEFI62117X0017).

Funding

This work was supported by the Russian Science Foundation, project no. 18-15-00360.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, ul. Pogodinskaya 10, bldg. 8, 119121, Moscow, Russia

    A. M. Gisina, Y. S. Kim, K. N. Yarygin & A. Yu. Lupatov

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  1. A. M. Gisina
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  2. Y. S. Kim
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  3. K. N. Yarygin
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  4. A. Yu. Lupatov
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Correspondence to A. M. Gisina.

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The authors declare that they have no conflict of interest. This article does not contain any research involving humans or animals as research objects.

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Translated by A. Medvedev

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Gisina, A.M., Kim, Y.S., Yarygin, K.N. et al. Identification of the Side Population Associated with ATP-Binding Cassette Transporters Activity Using Imaging Flow Cytometry. Biochem. Moscow Suppl. Ser. B 15, 248–254 (2021). https://doi.org/10.1134/S1990750821030033

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  • DOI: https://doi.org/10.1134/S1990750821030033

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