Skip to main content
SpringerLink
Log in

Establishment and Characterization of Human Colon-Cancer Cells Resistant to Cisplatin

  • Published:
Cell and Tissue Biology Aims and scope Submit manuscript

Abstract

An important stage of research devoted to the analysis of mechanisms of tumor recurrence and drug resistance is the generation of model lines of tumor cells that are resistant to the drug of interest. This paper describes the establishment of the cisplatin-resistant colon cancer cell line based on HCT116 colon cancer cells and characterizes some properties of resistant cells, including their proliferative, metabolic and migration activity. The resulting line HCT116/C exhibited more than 30-fold resistance to cisplatin compared to the original cells. As a result of our work, an in vitro model of colorectal-cancer cells resistant to cisplatin has been obtained. This model opens up opportunities for future research on overcoming drug resistance in vitro and search for new approaches to anticancer therapy in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

REFERENCES

  1. Boyer, J., McLean, E.G., Aroori, S., Wilson, P., McCulla, A., Carey, P.D., Longley, D.B., and Johnston, P.G., Characterization of p53 wild-type and null isogenic colorectal cancer cell lines resistant to 5-fluorouracil, oxaliplatin, and irinotecan, Clin. Cancer Res., 2004, vol. 10, p. 2158.

    Article  CAS  Google Scholar 

  2. Chen, P., Liu, X.-Q., Lin, X., Gao, L.-Y., Zhang, S., and Huang, X., Targeting YTHDF1 effectively re-sensitizes cisplatin-resistant colon cancer cells by modulating GLS-mediated glutamine metabolism, Mol. Ther. Oncolytics, 2021, vol. 20, p. 228.

    Article  CAS  Google Scholar 

  3. Choi, S.-U., Kim, N.-Y., Choi, E.-J., Kim, K.-H., and Lee, C.-O., Establishment of doxorubicin-resistant subline derived from hct15 human colorectal cancer cells, Arch. Pharm. Res., 1996, vol. 19, p. 342.

    Article  CAS  Google Scholar 

  4. Cui, Y., Qin, L., Tian, D., Wang, T., Fan, L., Zhang, P., and Wang, Z., ZEB1 promotes chemoresistance to cisplatin in ovarian cancer cells by suppressing SLC3A2, Chemotherapy, 2018, vol. 63, p. 262.

    Article  CAS  Google Scholar 

  5. Dasari, S. and Tchounwou, P.B., Cisplatin in cancer therapy: molecular mechanisms of action, Eur. J. Pharmacol., 2014, vol. 0, p. 364.

    Article  CAS  Google Scholar 

  6. Dilruba, S. and Kalayda, G.V., Platinum-based drugs: past, present and future, Cancer Chemother. Pharmacol., 2016, vol. 77, p. 1103.

    Article  CAS  Google Scholar 

  7. Durinikova, E., Kozovska, Z., Poturnajova, M., Plava, J., Cierna, Z., Babelova, A., Bohovic, R., Schmidtova, S., Tomas, M., Kucerova, L., and Matuskova, M., ALDH1A3 upregulation and spontaneous metastasis formation is associated with acquired chemoresistance in colorectal cancer cells, BMC Cancer, 2018, vol. 18, p. 848.

    Article  Google Scholar 

  8. Fanciulli, M., Bruno, T., Giovannelli, A., Gentile, F.P., Di Padova, M., Rubiu, O., and Floridi, A., Energy metabolism of human LoVo colon carcinoma cells: correlation to drug resistance and influence of lonidamine, Clin. Cancer Res., 2000, vol. 6, p. 1590.

    CAS  Google Scholar 

  9. Galluzzi, L., Senovilla, L., Vitale, I., Michels, J., Martins, I., Kepp, O., Castedo, M., and Kroemer, G., Molecular mechanisms of cisplatin resistance, Oncogene, 2012, vol. 31, p. 1869.

    Article  CAS  Google Scholar 

  10. Gupta, R.S., Murray, W., and Gupta, R., Cross resistance pattern towards anticancer drugs of a human carcinoma multidrug-resistant cell line, Br. J. Cancer, 1988, vol. 58, p. 441.

    Article  CAS  Google Scholar 

  11. Harker, W.G., Slade, D.L., Dalton, W.S., Meltzer, P.S., and Trent, J.M., Multidrug resistance in mitoxantrone-selected HL-60 leukemia cells in the absence of P-glycoprotein overexpression, Cancer Res., 1989, vol. 49, p. 4542.

    CAS  Google Scholar 

  12. Huang, H.-K., Lin, Y.-H., Chang, H.-A., Lai, Y.-S., Chen, Y.-C., Huang, S.-C., Chou, C.-Y., and Chiu, W.-T., Chemoresistant ovarian cancer enhances its migration abilities by increasing store-operated Ca2+ entry-mediated turnover of focal adhesions, J. Biomed. Sci., 2020, vol. 27, p. 36.

    Article  Google Scholar 

  13. Liu, Z., Qiu, M., Tang, Q.-L., Liu, M., Lang, N., and Bi, F., Establishment and biological characteristics of oxaliplatin-resistant human colon cancer cell lines, Chin. J. Cancer, 2010, vol. 29, p. 661.

    Article  CAS  Google Scholar 

  14. Michalak, M., Lach, M.S., Antoszczak, M., Huczyński, A., and Suchorska, W.M., Overcoming resistance to platinum-based drugs in ovarian cancer by salinomycin and its derivatives—an in vitro study, Molecules, 2020, vol. 25, p. 537.

    Article  CAS  Google Scholar 

  15. Schneider, C.A., Rasband, W.S., and Eliceiri, K.W., NIH image to imageJ: 25 years of image analysis, Nat. Methods, 2012, vol. 9, p. 671.

    Article  CAS  Google Scholar 

  16. Tang, H., Liu, Y.-J., Liu, M., and Li, X., Establishment and gene analysis of an oxaliplatin-resistant colon cancer cell line THC8307/L-OHP, Anticancer Drugs, 2007, vol. 18, p. 633.

    Article  CAS  Google Scholar 

  17. Vrie, W., Heyden, S.V.D., Gheuens, E., Bijma, A., Bruijn, E., Marquet, R., Oosterom, A., and Eggermont, A., Drug resistance in rat colon cancer cell lines is associated with minor changes in susceptibility to cytotoxic cells, Cancer Immunol. Immunother., 2005.

  18. Wu, Z.-X., Yang, Y., Zeng, L., Patel, H., Bo, L., Lin, L., and Chen, Z.-S., Establishment and characterization of an irinotecan-resistant human colon cancer cell line, Front. Oncol., 2021, vol. 10, p. 624954.

    Article  Google Scholar 

  19. Yang, L.Y. and Trujillo, J.M., Biological characterization of multidrug-resistant human colon carcinoma sublines induced/selected by two methods, Cancer Res., 1990, vol. 50, p. 3218.

    CAS  Google Scholar 

  20. Zhang, G., Fang, L., Zhu, L., Zhong, Y., Wang, P.G., and Sun, D., Syntheses and biological activities of 3'-azido disaccharide analogues of daunorubicin against drug-resistant leukemia, J. Med. Chem., 2006, vol. 49, p. 1792.

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the government of St. Petersburg and partly by the Foundation of the Director of the Institute of Cytology, Russian Academy of Sciences.

Author information

Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    A. V. Morshneva, O. O. Gnedina, D. N. Kindt & M. V. Igotti

Authors
  1. A. V. Morshneva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. O. Gnedina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. N. Kindt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. V. Igotti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Morshneva.

Ethics declarations

The authors declare no that they have no conflicts of interest. Experiments involving animals or human beings have not been performed.

Additional information

Translated by I. Fridlyanskaya

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Morshneva, A.V., Gnedina, O.O., Kindt, D.N. et al. Establishment and Characterization of Human Colon-Cancer Cells Resistant to Cisplatin. Cell Tiss. Biol. 16, 547–554 (2022). https://doi.org/10.1134/S1990519X22060037

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990519X22060037

Keywords:

Search

Navigation