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Proliferation of cultured glioma cells mediated by coenzyme Q10 under conditions of serum deprivation

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Abstract

The effect of coenzyme Q10 on glioma-cell proliferation under serum-deprived conditions has been studied. Our results have shown that the addition of coenzyme Q10 into a serum-free culture medium enhances cell viability, stimulates cell growth, restores mitochondrial potential, and increases the quantity of energized mitochondria. It is found that coenzyme Q10-induced glioma-cell proliferation in conditions of serum deficiency is a result of an intracellular reduced glutathione concentration with subsequent activation of protein kinase C, ERK1/2, and phosphoinositol-3-kinase.

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Abbreviations

ROS:

reactive oxygen species

EGF:

epidermal growth factor

FBS:

fetal bovine serum

GSH:

reduced glutathione

H2DCF-DA:

2,7-dichlorodihydrofluorescein diacetate

MCB:

monochlorobimane

PI3K:

phosphoinositol-3-kinase

PKC:

protein kinase C

S1P:

sphingosine-1-phosphate

SMPD:

sphingomyelin phosphodiesterase

SphK:

sphingosine kinase

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Authors and Affiliations

  1. Belarusian State University, Minsk, 220030, Belarus

    N. G. Krylova, T. A. Kulahava & G. N. Semenkova

  2. Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, 220114, Belarus

    S. V. Koran

Authors
  1. N. G. Krylova
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  2. T. A. Kulahava
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  3. S. V. Koran
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  4. G. N. Semenkova
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Correspondence to N. G. Krylova.

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Original Russian Text © N.G. Krylova, T.A. Kulahava, S.V. Koran, G.N. Semenkova, 2017, published in Tsitologiya, 2017, Vol. 59, No. 2, pp. 109–116.

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Krylova, N.G., Kulahava, T.A., Koran, S.V. et al. Proliferation of cultured glioma cells mediated by coenzyme Q10 under conditions of serum deprivation. Cell Tiss. Biol. 11, 220–226 (2017). https://doi.org/10.1134/S1990519X17030063

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  • DOI: https://doi.org/10.1134/S1990519X17030063

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