Abstract
Cyto- and genotoxic effects of usnic-acid (+)- and (–)-enantiomers have been studied in human peripheral-blood lymphocytes. It has been shown that usnic-acid enantiomers in concentrations of 0.04–0.30 mM have pronounced cytotoxic action. Usnic-acid enantiomers in concentrations of 0.04–0.30 mM have exhibited a genotoxic effect; moreover, the genotoxicity of usnic-acid (–)-enantiomer in concentrations of 0.15 and 0.30 mM was twice as high as that of (+)-enantiomer. The effect of usnic-acid (+)- and (–)-stereoisomers has been accompanied by the formation of atypical DNA comets. Furthermore, (–)-usnic acid has induced 2.5–3.5 times more atypical comets than its (+)-stereoisomer.
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Abbreviations
- DMSO:
-
dimethyl sulfoxide
- MMS:
-
methyl methanesulfonate
- ROS:
-
reactive oxygen species
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Original Russian Text © I.A. Prokopiev, E.V. Filippov, G.V. Filippova, N.P. Gladkina, 2017, published in Tsitologiya, 2017, Vol. 59, No. 1, pp. 13–18.
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Prokopiev, I.A., Filippov, E.V., Filippova, G.V. et al. Genotoxicity of usnic-acid enantiomers in vitro in human peripheral-blood lymphocytes. Cell Tiss. Biol. 11, 141–146 (2017). https://doi.org/10.1134/S1990519X17020031
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DOI: https://doi.org/10.1134/S1990519X17020031