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Intranuclear ubiquitin-immunopositive structures in human substantia nigra neurons

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Abstract

Marinesco bodies were discovered in substantia nigra neurons of human brain in 1902. The relationships between these intranuclear inclusions and the other structures of the cellular nucleus are still obscure. The aim of this study is to elucidate the morphological and cytochemical peculiarities of intranuclear ubiquitin-immunopositive bodies in the substantia nigra neurons of human brain and to evaluate the interconnections of these peculiarities with nucleolus by means of light microscopy, immunocytochemistry, and confocal laser microscopy. It is found that up to 20% of neurons in substantia nigra of human brain contain ubiquitin-immunopositive Marinesco bodies. These rounded structures are 1–8 μm—more often 2–4 μm—in diameter. Only one-third of them are tightly adjacent to the nucleolus. By a method of silver impregnation of argentophilic proteins associated with nucleolar organizer, the absence was shown of argentophilic proteins, which are characteristic for the nucleolus, in Marinesco bodies. Special ubiquitin-positive substantially smaller structures (less than 1 μm) are revealed in the neurons’ nuclei along with Marinesco bodies. These structures are probably the initial forms in the formation of Marinesco bodies. The existence of two types of ubiquitin-immunopositive intranuclear bodies is revealed by means of confocal microscopy: one has high intensity of immunofluorescence, and the other has low intensity. Heterogeneous distribution of immunopositive product is characteristic of the former. The presence of DNA in Marinesco bodies is detected by using SYTOX Green fluorescent dye. The absence of peripheral heterochromatin zone and weak susceptibility to toluidine blue together with the presence of DNA and the absence of argentophilic proteins suggests substantial structural and chemical differences between Marinesco bodies and nucleoli, which argues against the idea that the detected bodies are modified nucleoli.

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Authors and Affiliations

  1. Institute of Experimental Medicine, 12 ul. Akademika Pavlova, St. Petersburg, 197376, Russia

    I. P. Grigor’ev, D. E. Korzhevskii, E. G. Sukhorukova, V. V. Gusel’nikova & O. V. Kirik

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  1. I. P. Grigor’ev
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  2. D. E. Korzhevskii
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  3. E. G. Sukhorukova
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  4. V. V. Gusel’nikova
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  5. O. V. Kirik
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Correspondence to I. P. Grigor’ev.

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Original Russian Text © I.P. Grigor’ev, D.E. Korzhevskii, E.G. Sukhorukova, V.V. Gusel’nikova, O.V. Kirik, 2015, published in Tsitologiya, 2015, Vol. 57, No. 11, pp. 780–787.

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Grigor’ev, I.P., Korzhevskii, D.E., Sukhorukova, E.G. et al. Intranuclear ubiquitin-immunopositive structures in human substantia nigra neurons. Cell Tiss. Biol. 10, 29–36 (2016). https://doi.org/10.1134/S1990519X16010053

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