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Dexamethasone Modulates Lipopolysaccharide-Induced Expression of Proinflammatory Cytokines in Rat Hippocampus

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Abstract—

The effects of intrahippocampal injection of dexamethasone on the expression of proinflammatory cytokines in the dorsal and ventral parts of the hippocampus were studied. We evaluated mRNA expression of interleukin-1b (Il1b), interleukin-6 (Il6), and tumor necrosis factor (Tnf) 3 days after injection of dexamethasone (DEX), bacterial lipopolysaccharide (LPS), and their combination in the dorsal hippocampus. Phosphate buffered saline administered to control animals did not affect the expression of the cytokines studied. In the dorsal hippocampus, LPS induced a significant increase in the level of Tnf and a trend to an increase in Il1b and Il6 mRNA expression. DEX alone induced a trend to an increase in the expression of Il1b. A DEX + LPS combination also tended to increase Il1b expression and a significant increase in Tnf. In addition, we observed a considerable decrease in Il6 mRNA level after DEX + LPS as compared to LPS group. In the ventral hippocampus, we observed a trend to an increase in the level of Il1b after DEX + LPS and a trend to an increase in Tnf after DEX alone. The data suggest that DEX by itself is able to induce weak neuroinflammation in the hippocampal tissue, and, when applied during LPS-induced neuroinflammation, evokes differential effects on proinflammatory cytokines expression indicating a complex DEX-mediated regulation of cytokine balance in the hippocampus.

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Funding

The reported study was funded by the RFBR, project no. 19-315-90081.

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, ul. Butlerova 5a, 117485, Moscow, Russia

    L. V. Tret’yakova, A. A. Kvichansky, A. P. Bolshakov & N. V. Gulyaeva

  2. Research and Clinical Center for Neuropsychiatry of Moscow Healthcare Department, Moscow, Russia

    N. V. Gulyaeva

Authors
  1. L. V. Tret’yakova
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  2. A. A. Kvichansky
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  3. A. P. Bolshakov
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  4. N. V. Gulyaeva
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Correspondence to N. V. Gulyaeva.

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Conflicts of interest. The authors declare that they have no conflicts of interest.

Ethical approval. All experiments were conducted in compliance with EU Directive 2010/63/EU for animal experiments and the National Institutes of Health guide for the care and use of Laboratory animals (NIH Publications No. 8023, revised 1978) and the Ethics Committee of Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences (protocol number 10, December 10, 2012).

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Tret’yakova, L.V., Kvichansky, A.A., Bolshakov, A.P. et al. Dexamethasone Modulates Lipopolysaccharide-Induced Expression of Proinflammatory Cytokines in Rat Hippocampus. Neurochem. J. 15, 302–307 (2021). https://doi.org/10.1134/S1819712421330011

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  • DOI: https://doi.org/10.1134/S1819712421330011

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