Abstract
The effect of carbon ions (12C) with the energy of 400 MeV/nucleon on the dynamics of induction and growth rate of solid tumors in mice under irradiation of Ehrlich ascites carcinoma cells (EAC) ex vivo at doses of 5–30 Gy relative to the action of equally effective doses of X-ray radiation was studied. The dynamics of tumor induction under the action of 12C and X-rays had a similar character and depended on the dose during 3 months of observation. The value of the latent period, both when irradiating cells with 12C and X-ray, increased with increasing dose, and the interval for tumor induction decreased. The rate of tumor growth after ex vivo irradiation of EAC cells was independent of either dose or type of radiation. The dose at which EAC tumors are not induced within 90 days was 30 Gy for carbon ions and 60 Gy for X-rays. The value of the relative biological effectiveness of carbon ions, calculated from an equally effective dose of 50% probability of tumors, was 2.59.
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ACKNOWLEDGMENTS
We express our deep gratitude to the employees of the Laboratory of the Ion Beam Complex of the National Research Center “Kurchatov Institute” V.A. Pikalov and A.G. Vasil’eva for assistance in organizing and conducting experiments on the equipment of the Radiobiological Stand on a Carbon Beam U-70.
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The work was carried out under of the State assignment of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences no. 075-01025-23-01 and with partial funding under the research agreement no. 28/22 between the Branch “Physical and Technical Center” of the Lebedev Physical Institute of the Russian Academy of Sciences and the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences.
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Animal experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (http://oacu.od.nih.gov/regs/index.htm). Protocols for animal experiments were approved by the Commission on Biological Safety and Bioethics of the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences (no. 11/2023 dated August 2, 2023).
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Balakin, V.E., Belyakova, T.A., Rozanova, O.M. et al. Anti-tumor Effect of High Doses of Carbon Ions and X-Rays during Irradiation of Ehrlich Ascites Carcinoma Cells Ex Vivo. Dokl Biochem Biophys 513 (Suppl 1), S30–S35 (2023). https://doi.org/10.1134/S1607672924700765
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DOI: https://doi.org/10.1134/S1607672924700765