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Sfmbt Co-purifies with Hangover and SWI/SNF-Remodelers in Drosophila melanogaster

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Polycomb group (PcG) proteins are chromatin-associated factors involved in the repression of gene transcription. In the present study, we characterized the interactome of the Sfmbt factor at the embryonic stage of development. For this, the Sfmbt protein complex was affinity purified from the nuclear extract, followed by highly specific peptide sequencing (IP/LC-MS). As a result, a number of previously uncharacterized Sfmbt interactions were discovered. In particular, Sfmbt top-interacting proteins include the DNA-binding protein Hangover and components of the SWI/SNF family of chromatin remodelers.

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ACKNOWLEDGMENTS

This study was performed using the infrastructure of the Core Facility of the Institute of Gene Biology, Russian Academy of Sciences and the Center for High-Precision Editing and Genetic Technologies for Biomedicine of the Institute of Gene Biology, Russian Academy of Sciences.

Funding

This work was supported by the Russian Science Foundation (project no. 18-74-10091).

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

    M. M. Erokhin, Y. V. Shidlovskii, D. V. Lomaev,  P. G. Georgiev & D. A. Chetverina

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  1. M. M. Erokhin
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  2. Y. V. Shidlovskii
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  3. D. V. Lomaev
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  4. P. G. Georgiev
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  5. D. A. Chetverina
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Corresponding author

Correspondence to D. A. Chetverina.

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Translated by M. Batrukova

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Erokhin, M.M., Shidlovskii, Y.V., Lomaev, D.V. et al. Sfmbt Co-purifies with Hangover and SWI/SNF-Remodelers in Drosophila melanogaster. Dokl Biochem Biophys 500, 304–307 (2021). https://doi.org/10.1134/S1607672921050069

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  • DOI: https://doi.org/10.1134/S1607672921050069

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