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Enzymatic Degradation of Capsules Based on Polyelectrolyte Polypeptide–Polysaccharide Complex for the Controlled Release of DNA

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Abstract

DNA-containing biodegradable microcapsules based on vaterite CaCO3 polycrystalline particles and polyelectrolyte complex polyarginine–dextran sulfate are obtained and characterized. The enzymatic degradation of the microcapsules induced by pronase is studied using scanning electron microscopy, laser confocal microscopy, and spectrophotometry. It is shown that microcapsules the shell of which is formed by polypeptide–polysaccharide polyelectrolyte complexes are sensitive to pronase-induced enzymatic hydrolysis leading to the release of encapsulated DNA.

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Funding

This work was supported by the Ministry of Science and Higher Education within the framework of the State Assignment for the Crystallography and Photonics Federal Research Center, Russian Academy of Sciences, using equipment of the Shared Research Center, Crystallography and Photonics Federal Research Center, Russian Academy of Sciences, in terms of obtaining microcapsules and their characterization and by the National Research Center Kurchatov Institute (no. 1058 dated July 2, 2020) in terms of the study of the enzymatic biodegradation of capsules.

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Authors and Affiliations

  1. Crystallography and Photonics Federal Research Center, Russian Academy of Sciences, 119333, Moscow, Russia

    T. N. Borodina, D. A. Shepelenko, D. B. Trushina, V. V. Artemov & T. V. Bukreeva

  2. First Moscow State Medical University, 119991, Moscow, Russia

    D. B. Trushina

  3. National Research Center Kurchatov Institute, 123098, Moscow, Russia

    T. V. Bukreeva

Authors
  1. T. N. Borodina
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  2. D. A. Shepelenko
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  3. D. B. Trushina
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  4. V. V. Artemov
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  5. T. V. Bukreeva
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Correspondence to T. N. Borodina.

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Borodina, T.N., Shepelenko, D.A., Trushina, D.B. et al. Enzymatic Degradation of Capsules Based on Polyelectrolyte Polypeptide–Polysaccharide Complex for the Controlled Release of DNA. Polym. Sci. Ser. B 63, 514–520 (2021). https://doi.org/10.1134/S156009042105002X

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  • DOI: https://doi.org/10.1134/S156009042105002X

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