Abstract
MicroRNAs (miRNAs) are a kind of small non-coding RNA molecules that regulate gene expression by targeting mRNA and play critical roles involved various life processes of organisms. Batrachuperus tibetanus (B. tibetanus) as an endangered amphibian species of salamander endemic to China, has attracted much attention because of its value of studying paleontology evolutionary history and decreasing population size. Until now, research in gonadal miRNA of B. tibetanus have not been reported. In this study, RNA sequencing technology was used to screen miRNAs based on the construction of two cDNA libraries from adult testis and ovary tissues of B. tibetanus, respectively. Totally, 15 481 068 and 15 929 246 raw reads were yielded in testis and ovary, respectively, representing five known and 88 novel miRNAs. Among all these identified miRNA, 54 differentially expressed miRNAs were found between testis and ovary of B. tibetanus containing 25 up-regulated as well as 29 down-regulated miRNAs. Ten miRNAs were randomly chosen to verify their expression by using stem-loop qRT-PCR indicating the reliability and accuracy of sequenced data. Additionally, further analysis of target mRNAs of differentially expressed miRNAs reveals that they were mainly involved in cellular processes, reproduction and reproductive process related GO terms. Our current result is the first to perform the identification, characterization, differential expression and functional analysis of gonadal miRNAs both in testis and ovary of B. tibetanus, which also provide a valuable reference to enhance the resource protection of stream salamander species.
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The project was supported by Guangdong Province and the Key Research Project of Higher Education Institutions of Henan Province (23A240003).
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Tan, S., Huang, Y., Xiong, J. et al. Identification and Comparative Analysis of the miRNAs in Gonads of High-altitude Species, Batrachuperus tibetanus. Russ J Bioorg Chem 48 (Suppl 1), S144–S153 (2022). https://doi.org/10.1134/S1068162023010260
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DOI: https://doi.org/10.1134/S1068162023010260