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Detection of the PRAME Protein on the Surface of Melanoma Cells Using a Fluorescently Labeled Monoclonal Antibody

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Abstract

Determination of the expression level of tumor marker PRAME protein is important both for predicting the course of the disease and for monitoring the effectiveness of anticancer therapy. A fluorescently labeled monoclonal antibody to the PRAME protein was obtained by periodate oxidation of glycans followed by modification with a bifunctional azido-oxyamine reagent and a “click” reaction with alkyne-modified sulfonated cyanine dye Cy3. A new approach to the synthesis of a bifunctional azido-oxyamine reagent using the ethoxyethylidene protecting group for oxyamine is proposed. The labeled antibodies were characterized with UV/Vis absorption spectra and the stoichiometry of the modification was determined. It has been demonstrated that the fluorescent antibodies retain affinity and can be used as a diagnostic tool for detecting the residual marker (the PRAME protein) after anticancer therapy.

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ACKNOWLEDGMENTS

The authors are grateful to the student of the Department of Biology, Moscow State University, E.A. Kokin, for conducting preliminary experiments on modification of model oxidized polyclonal antibodies with an oxyamine reagent (I).

Funding

The development of a method for modifying monoclonal antibodies was supported by the Russian Science Foundation (project 20-15-00361). K.A. Sapozhnikova is grateful for the financial support from the Russian Foundation for Basic Research (project 20-34-90125 “Postgraduates”).

Author information

Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, 117997, Moscow, Russia

    K. A. Sapozhnikova, E. G. Meleshkina, S. D. Oreshkov, E. P. Ganzhula, A. S. Mikhailova, V. A. Korshun & V. A. Brylev

  2. GeneTechnology LLC, 117485, Moscow, Russia

    A. V. Misyurin

  3. Moscow Institute of Physics and Technology, 141701, Moscow, Russia

    N. B. Pestov

  4. Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, 108819, Poselenie Moskovskii, Russia

    N. B. Pestov

  5. Mendeleev University of Chemical Technology of Russia, 125047, Moscow, Russia

    E. G. Meleshkina

  6. Moscow State University, Department of Chemistry, 119991, Moscow, Russia

    S. D. Oreshkov

  7. Peoples’ Friendship University of Russia, 117198, Moscow, Russia

    E. P. Ganzhula

  8. Higher School of Economics, Department of Biology and Biotechnology, 117312, Moscow, Russia

    A. S. Mikhailova

  9. Gause Institute of New Antibiotics, 119021, Moscow, Russia

    V. A. Korshun

  10. N.N. Blokhin National Medical Research Center of Oncology, 115478, Moscow, Russia

    V. A. Misyurin

Authors
  1. K. A. Sapozhnikova
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  2. A. V. Misyurin
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  3. N. B. Pestov
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  4. E. G. Meleshkina
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  5. S. D. Oreshkov
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  6. E. P. Ganzhula
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  7. A. S. Mikhailova
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  8. V. A. Korshun
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  9. V. A. Misyurin
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  10. V. A. Brylev
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Corresponding authors

Correspondence to V. A. Misyurin or V. A. Brylev.

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COMPLIANCE WITH ETHICAL STANDARDS

This article does not contain a description of experiments conducted by any of the authors of this article with the participation of humans or the use of animals as research objects.

CONFLICT OF INTEREST

The authors declare they have no conflicts of interest.

Additional information

Abbreviations: Boc, tert-butyloxycarbonyl; ESI, electrospray ionization; PRAME, preferentially expressed antigen in melanoma; THPTA, tris(3-hydroxypropyltriazolylmethyl)amine.

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Sapozhnikova, K.A., Misyurin, A.V., Pestov, N.B. et al. Detection of the PRAME Protein on the Surface of Melanoma Cells Using a Fluorescently Labeled Monoclonal Antibody. Russ J Bioorg Chem 47, 1077–1085 (2021). https://doi.org/10.1134/S1068162021050332

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