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Novel fluorescent membrane probe 2,3;5,6-bis(cyclohexyl)-BODIPY-labeled phosphatidylcholine

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Abstract

The novel fluorescent membrane probe, bis(cyclohexyl)-BODIPY (BCHB)-labeled phosphatidylcholine, is structurally similar to 1,3,5,7-tetramethyl-BODIPY (TMB)-labeled phosphatidylcholine. Formally, BCHB and TMB have similar systems of conjugated bonds; however, spectral properties of the probes are notably different. BCHB and TMB have a perfect spectral overlap. The fact makes BCHB a good FRET acceptor for TMB. Thus, the pair of phosphatidylcholines labeled with BCHB and TMB is a good tool for FRET-based membrane studies, e.g. lipid transfer studies.

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Abbreviations

Ag-LTP:

Anethum graveolens lipid transfer protein

BODIPY:

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

BCHB:

2,3;5,6-bis(cyclohexyl)-BODIPY

FRET:

Forster resonance energy transfer

TMB:

1,3,5,7-tetramethyl-BODIPY

LTP:

lipid transfer protein

PC:

phosphatidylcholine

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    A. S. Alekseeva, D. S. Tretiakova, D. N. Melnikova, Ul. G. Molotkovsky & I. A. Boldyrev

  2. Pirogov Russian National Research Medical University, ul. Ostrovityanova 1, 117997, Moscow, Russia

    I. A. Boldyrev

Authors
  1. A. S. Alekseeva
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  2. D. S. Tretiakova
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  3. D. N. Melnikova
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  4. Ul. G. Molotkovsky
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  5. I. A. Boldyrev
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Corresponding author

Correspondence to I. A. Boldyrev.

Additional information

Original Russian Text © A.S. Alekseeva, D.S. Tretiakova, D.N. Melnikova, Ul.G. Molotkovsky, I.A. Boldyrev, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 3, pp. 339–344.

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Alekseeva, A.S., Tretiakova, D.S., Melnikova, D.N. et al. Novel fluorescent membrane probe 2,3;5,6-bis(cyclohexyl)-BODIPY-labeled phosphatidylcholine. Russ J Bioorg Chem 42, 305–309 (2016). https://doi.org/10.1134/S1068162016030031

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  • DOI: https://doi.org/10.1134/S1068162016030031

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