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Radiosynthesis, In Vitro and In Vivo Evaluation of 99mTc-Silodosin as a Tumor Marker in Mice

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Radiochemistry Aims and scope

Abstract

To obtain a candidate tumor-imaging agent, Silodosin was labeled with technetium-99m. The 95% radiochemical yield was reached under the following conditions: 100 µg of Silodosin, 100 µg of SnCl2·2H2O, 100 µL of buffer solution (pH 4), 80°C, 15 min. The biodistribution of 99mTc-Silodosin in normal and tumored mice revealed the potential for using the tracer to diagnose the solid tumor. The tumored/nontumored muscle ratio was 3.33 at 60 min post-injection, indicating high affinity of 99mTc-Silodosin for the solid tumor. The in vitro uptake of the tracer by PC-3 cells revealed the ability of 99mTc-Silodosin to conjugate with the adrenoceptor found in the prostate.

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ACKNOWLEDGMENTS

The authors are grateful to Assistant Prof. Ahmed Rashad for his valuable help and support in completing this paper.

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Authors and Affiliations

  1. Cyclotron Project, Nuclear Research Centre, Egyptian Atomic Energy Authority, 13759, Cairo, Egypt

    Marwa S. El Refaye, S. I. Khater, H. M. Essam & H. Aglan

  2. Radioactive Isotopes and Generators Department, Hot Laboratories Center, Egyptian Atomic Energy Authority, 13759, Cairo, Egypt

    Marwa S. El Refaye & S. I. Khater

  3. Biological Application Department, Nuclear Research Center, Egyptian Atomic Energy Authority, 13759, Cairo, Egypt

    H. M. Essam

  4. Labeled Compounds Department, Hot Laboratories Center, Egyptian Atomic Energy Authority, 13759, Cairo, Egypt

    H. Aglan

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  1. Marwa S. El Refaye
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  2. S. I. Khater
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Correspondence to Marwa S. El Refaye.

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Refaye, M.S.E., Khater, S.I., Essam, H.M. et al. Radiosynthesis, In Vitro and In Vivo Evaluation of 99mTc-Silodosin as a Tumor Marker in Mice. Radiochemistry 63, 828–834 (2021). https://doi.org/10.1134/S1066362221060151

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