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Biosynthetic potential of actinomycetes in brown forest soil on the eastern coast of the aegean sea

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Abstract

The taxonomic and functional structures of the actinomycetal complex in the litter and upper horizon of the brown forest soil was studied in a Pinus brutia var. pendulifolia forest on the eastern coast of the Aegean Sea. The complex of actinomycetes included representatives of the Streptomyces and Micromonospora genera and oligosporus forms. Streptomycetes predominated (73.8%) in the soil, and micromonospores (66.7%) were dominants in the litter. Thirty isolates of ten Streptomyces species from five series and three sections prevailed. In the upper soil horizon, species of the Helvolo-Flavus Helvolus section predominated (48%); the S. felleus species occurred most frequently. Among the isolated cultures, the S. globisporus and S. sindenensis species capable to produce antitumor antibiotics were found. The testing of the antimicrobial activity of the natural isolates showed that five strains inhibit the growth of pathogenic Fusarium sp., Alternaria sp., Acremonium sp., and Bipolaris sorokiniana fungi. When testing the effect of streptomycetes on the production of cellulases, a high-efficient strain belonging to the S. noboritoensis species was revealed. All the streptomycetes isolated from the brown forest soil produced auxins at the rate of 7.8 to 19.7 μg of indole acetic acid/mL of the liquid medium in the presence of 200 mg/L of tryptophan. Twelve isolates of streptomycetes were transferred to the collection of biotechnologically promising cultures for studying their properties.

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Authors and Affiliations

  1. Rudnitsky Zonal Research Institute of Agriculture in the Northeast, Kirov, 610007, Russia

    I. G. Shirokikh & A. A. Shirokikh

  2. Institute of Biology, Komi Science Center, Ural Branch of the Russian Academy of Sciences and Vyatka State University, Kirov, 610002, Russia

    I. G. Shirokikh & A. A. Shirokikh

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  1. I. G. Shirokikh
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  2. A. A. Shirokikh
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Correspondence to I. G. Shirokikh.

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Original Russian Text © I.G. Shirokikh, A.A. Shirokikh, 2017, published in Pochvovedenie, 2017, No. 11, pp. 1335–1361.

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Shirokikh, I.G., Shirokikh, A.A. Biosynthetic potential of actinomycetes in brown forest soil on the eastern coast of the aegean sea. Eurasian Soil Sc. 50, 1311–1317 (2017). https://doi.org/10.1134/S1064229317110114

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