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The Proliferation of Cherry Dwarf Rootstocks: The Effects of Nutrient Media, Carbon Sources, and Genetic Fidelity Evaluation Using Simple Sequence Repeat Markers

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Abstract

In this study, three types of culture media, including Murashige and Skoog (MS), woody plant medium (WPM), and Quoirin and Lepoivre (LP) medium fortified with 0, 1, 2, and 3 mg/L of N6-benzyladenine (BA) and 0, 0.25, and 0.50 mg/L thidiazuron (TDZ) were investigated. Specifically, this study aimed to determine these culture media’s effects on the rate of explants establishment, shoot multiplication, and shoot length of cherry dwarf rootstocks Gisela 12 and Maxma 14. After finding the best treatment culture medium and plant growth regulators in the first experiment, different carbon sources (Sucrose, glucose, fructose, and sorbitol, separately) at three concentrations (15, 30, and 45 g/L) were evaluated to improve the results. The regenerated plants and mother plant were surveyed with 12 simple sequence repeat primers for the uniformity test. The highest establishment rate (100%) was obtained in the woody plant medium containing 3 mg/L BA, along with 0.25, 0.5, and 1.0 mg/L TDZ in both cherry dwarf rootstocks. The highest shoot proliferation rates (16.5 in Gisela 12 and 9.3 in Maxma 14) were recorded for the woody plant medium enriched with 45 g/L sorbitol and 2 mg/L BA + 0.5 mg/L TDZ. A simple sequence repeat marker analysis confirmed the genetic uniformity of the micropropagated plantlets and showed that this protocol could be a reliable method for the efficient in vitro reproduction of cherry dwarf rootstocks on a large scale.

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ACKNOWLEDGMENTS

The financial support of the Islamic Azad University of Abhar, Abhar, Iran, is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Horticultural Science, Abhar Branch, Islamic Azad University, Abhar, Iran

    Ali Mehrban Jafarlou, Saeed Piri Pirivatlo & Behrooz Salehi

  2. Department of Molecular Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran

    Amir Hoshang Hoseynzadeh Mogbli

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  1. Ali Mehrban Jafarlou
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  2. Saeed Piri Pirivatlo
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  3. Behrooz Salehi
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  4. Amir Hoshang Hoseynzadeh Mogbli
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Correspondence to Saeed Piri Pirivatlo.

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This article does not contain any studies involving animals or human participants performed by any of the authors.

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Ali Mehrban Jafarlou, Pirivatlo, S.P., Salehi, B. et al. The Proliferation of Cherry Dwarf Rootstocks: The Effects of Nutrient Media, Carbon Sources, and Genetic Fidelity Evaluation Using Simple Sequence Repeat Markers. Biol Bull Russ Acad Sci 49 (Suppl 2), S102–S112 (2022). https://doi.org/10.1134/S1062359022140084

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