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Microbial population of the digestive tract of click beetle larvae (Elateridae, Coleoptera)

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Abstract

The composition and functional structure of the intestinal microflora of three wireworm species (Agriotes obscurus (L.), Selatosomus aeneus (L.), and Ampedus pomorum (Herbst)) with different dietary regimes were studied. The total abundance of the microorganisms was evaluated by fluorescent microscopy, the group composition was assessed by inoculation on a solid glucose-peptone-yeast medium, and the functional diversity was estimated by multisubstrate testing. It was noted that, in the intestine of the larvae, the total number of microorganisms was lower by 1–2 orders of magnitude than in the soil and decaying wood. It was found that the composition of the intestinal microbial communities of wireworms was radically different from that of the substrate: the Bray-Curtis coefficient did not exceed 0.25. It was found that native forms accounted for more than half of the total number of saprotrophic bacteria: in the larvae, Gram-positive cocci, enterobacteria, Vibrionaceae, Acinetobacter, and some genera of coryneform bacteria, which were absent in the soil and wood, prevailed. The micromycetes were either absent (Agriotes) or were found in insignificant quantities (Selatosomus, Ampedus). In Selatosomus, apart from the intestinal forms, representatives of Mezorhizobium, Nocardioides, and Erwinia, occurring on plant substrates, were observed.

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Authors and Affiliations

  1. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia

    E. S. Samoilova & B. R. Striganova

  2. Faculty of Soil Science, Moscow State University, Leninskie gory, Moscow, 119991, Russia

    N. V. Kostina

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  1. E. S. Samoilova
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  2. N. V. Kostina
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  3. B. R. Striganova
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Correspondence to B. R. Striganova.

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Original Russian Text © E.S. Samoilova, N.V. Kostina, B.R. Striganova, 2016, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2016, No. 5, pp. 532–543.

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Samoilova, E.S., Kostina, N.V. & Striganova, B.R. Microbial population of the digestive tract of click beetle larvae (Elateridae, Coleoptera). Biol Bull Russ Acad Sci 43, 457–467 (2016). https://doi.org/10.1134/S1062359016050083

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