Abstract
A parallel analysis of the macrosamples of surrounding soil and microsamples of rhizosphere soil did not reveal the so-called rhizospheric effect. The data obtained showed that dilution significantly influences the results of determination of the number of soil microorganisms. The actual number of microorganisms revealed in soil samples greatly differed from the theoretically predicted values. The enumeration of microorganisms in soil microsamples by direct count and, especially, by the plating method with the use of conversion coefficients based on the degree of sample dilution gave erroneous results.
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Polyanskaya, L.M., Orazova, M.K., Burkanova, O.A. et al. Microsampling of rhizosphere soil and laboratory artifacts. Microbiology 69, 486–489 (2000). https://doi.org/10.1007/BF02756775
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DOI: https://doi.org/10.1007/BF02756775