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Voltammetric Determination of 7-Ethylthio-3-Nitro-1,2,4-Triazolo[5,1-c]-1,2,4-Triazin-4-One Dihydrate Sodium Salt as a Potential Antiviral Drug

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Abstract

The sodium salt of 7-ethylthio-3-nitro-1,2,4-triazolo[5,1-c]-1,2,4-triazin-4-one dihydrate (1) is one of promising antiviral compounds that have shown a pronounced biological activity against influenza virus subtype H5N1, West Nile, and SARS (severe acute respiratory syndrome). Using cyclic voltammetry, it was shown that the electrochemical activity of compound 1 in a Britton–Robinson buffer solution (BRB) of pH 2.0 on a glassy carbon electrode is due to the electrochemical reduction of the nitro group conjugated with the heterocyclic system. A method was developed for the determination of compound 1 by direct cathodic square-wave voltammetry. The linearity region of the calibration curve in a BRB solution of pH 7.0 is 10–300 mg/L (R2 = 0.999), the limit of detection is 1.5 mg/L, and the limit of quantification is 4.5 mg/L. The accuracy of the developed procedure is close to 100%, the relative standard deviation is 3.6%.

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Funding

The work was supported by the Ministry of Science and Higher Education of the Russian Federation, State Contract no. FEUZ-2023-0021 (N687.42B.325/23).

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Authors and Affiliations

  1. Ural Federal University named after the first President of Russia B.N. Yeltsin, 620002, Yekaterinburg, Russia

    P. N. Mozharovskaia, A. V. Ivoilova, T. V. Dem’yanovich (Selyanina), I. A. Balin, N. A. Malakhova, A. V. Ivanova, A. N. Kozitsina, A. I. Matern & V. L. Rusinov

  2. I.Ya. Postovsky Institute of Organic Synthesis of the Ural Branch of the Russian Academy of Sciences, 620137, Yekaterinburg, Russia

    V. L. Rusinov

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  1. P. N. Mozharovskaia
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  2. A. V. Ivoilova
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  3. T. V. Dem’yanovich (Selyanina)
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  4. I. A. Balin
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  5. N. A. Malakhova
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  6. A. V. Ivanova
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  7. A. N. Kozitsina
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  8. A. I. Matern
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  9. V. L. Rusinov
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Correspondence to P. N. Mozharovskaia.

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Translated by V. Kudrinskaya

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Mozharovskaia, P.N., Ivoilova, A.V., Dem’yanovich (Selyanina), T.V. et al. Voltammetric Determination of 7-Ethylthio-3-Nitro-1,2,4-Triazolo[5,1-c]-1,2,4-Triazin-4-One Dihydrate Sodium Salt as a Potential Antiviral Drug. J Anal Chem 78, 1688–1693 (2023). https://doi.org/10.1134/S1061934823120122

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