Abstract
A method of liquid–liquid microextraction is proposed for the extraction and preconcentration of tetracycline antibiotics from milk. It involves the extraction of analytes into a hydrophobic deep eutectic solvent (DES) based on a terpenoid and a carboxylic acid, followed by back extraction into the aqueous phase. A synergistic effect was revealed in the microextraction of tetracyclines into a DES phase based on thymol and octanoic acid (molar ratio of precursors 0.35 : 0.65), i.e., higher distribution ratios compared to those calculated using the additive scheme. Possibilities of the proposed method are shown on an example of the determination of tetracycline, chlortetracycline, and doxycycline in milk samples by HPLC–MS/MS. The limits of detection (3σ) for tetracycline and doxycycline are 5 ng/kg and for chlortetracycline,—50 ng/kg.
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REFERENCES
Grayson, M.L., Crowe, S.M., McCarthy, J.S., Mills, J., Mouton, J.W., Norrby, S.R., and Pfaller, M.A., Kucers’ The Use of Antibiotics, BocaRaton: CRC, 2010, 6th ed., p. 3078.
Michalova, E., Vet. Med., 2004, vol. 49, no. 3, p. 79
Companyo, R., Anal. Bioanal. Chem., 2009, vol. 395, no. 4, p. 877.
Dmitrienko, S.G., Apyari, V.V., Tolmacheva, V.V., and Gorbunova, M.V., J. Anal. Chem., 2020, vol. 75, no. 10, p. 1237.
Dmitrienko, S.G. Apyari, V.V., Gorbunova, M.V., Tolmacheva, V.V., and Zolotov, Yu.A., J. Anal. Chem., 2020, vol. 75, no. 10, p. 1371.
Shishov, A., Shishov, A., Pochivalov, A., Nugbienyo, L., Andruch, V., and Bulatov, A., TrAC, Trends Anal. Chem., 2020, vol. 129, 115956.
Smith, E.L., Abbott, A.P., and Ryder, K.S., Chem. Rev., 2014, vol. 114, no. 21, p. 11060.
Kudlak, B., Owczarek, K., and Namiesnik, J., Environ. Sci. Pollut. Res., 2015, vol. 22, no. 16, p. 11975.
Ozturk, B., Esteban, J., and Gonzalez-Miquel, M., J. Chem. Eng. Data, 2018, vol. 63, no. 7, p. 2384.
Shishov, A., Terno, P., Moskvin, L., and Bulatov, A.A., Talanta, 2020, vol. 206, 120209.
Shishov, A., Volodina, N., Nechaeva, D., Gagarinova, S., and Bulatov, A., RSC Adv., 2018, vol. 67, p. 38146.
Nedaei, M., Zarei, A.R., and Ghorbanian, S.A., New J. Chem., 2018, vol. 42, no. 15, p. 12520.
Sereshti, H., Jazani, S.S., Nouri, N., and Shams, G., Microchem. J., 2020, vol. 158, 105269.
Cherkashina, K., Pochivalov, A., Simonova, V., Shakirova, F., Shishov, A., Bulatov, A., Analyst, 2021, vol. 146, no. 11, p. 3449.
Technical Regulations of the Customs Union TR TS 021/2011.
Korenman, I.M., Ekstraktsiya v analize organicheskikh veshchestv (Extraction in the Analysis of Organic Substances) Moscow: Khimiya, 1977.
Martins, M.A.R., Crespo, E.A., Pontes, P.V., Silva, L.P., Bülow, M., Maximo, G.J., and Coutinho, J.A., ACS Sustainable Chem. Eng., 2018, vol. 6, no. 7, p. 8836.
Colaizzi, J.L. and Klink, P.R., J. Pharm. Sci., 1969, vol. 58, no. 10, p. 1184.
Abbasi, M.M., Babaei, H., and Ansarin, M., Adv. Pharm. Bull., 2011, vol. 1, no. 1, p. 34.
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The authors are grateful to the staff of the Research Park of St. Petersburg State University “Center for Chemical Analysis and Materials Research.”
Funding
This work was supported by the Russian Science Foundation, project no. 21-13-00020 (https://rscf.ru/project/21-13-00020/) and also by the Russian Foundation for Basic Research, project no. 19-33-90007.
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Cherkashina, K.D., Pochivalov, A.S., Shakirova, F.M. et al. Microextraction of Tetracyclines from Milk to Deep Eutectic Solvents for the Subsequent Determination by High-Performance Liquid Chromatography–Tandem Mass Spectrometry. J Anal Chem 77, 334–341 (2022). https://doi.org/10.1134/S1061934822030042
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DOI: https://doi.org/10.1134/S1061934822030042