Abstract
A new spectrofluorimetric method is described for the determination of uric acid (UA), that can remarkably reduce the fluorescence intensity of the enoxacin (ENX)-terbium ion (Tb3+) complex at 545 nm. The reduced fluorescence intensity of Tb3+ ion at pH 5.7 is proportional to the concentration of UA. Optimum conditions for the determination of UA have been investigated. The linear range and detection limit for the determination of UA are 6.0 × 10−7–3.0 × 10−5 M and 1 × 10−7 M, respectively. The relative standard deviation (RSD) was 0.4% for 6 × 10−6 M UA (n = 11). The method is simple, practical and relatively free of interferences. It has been successfully applied to assess UA in serum at the level of 3 × 10−4 M with an RSD of 5–7% (n = 3). The results were evaluated by comparison with a common clinical spectrophotometric method using phosphotungstic acid as developer.
Similar content being viewed by others
References
Grabowska, I., Chudy, M., Dybko, A., and Brzozka, Z. Sens. Actuat. B, 2008, vol. 130, no. 1, p. 508.
Ye, Y.W. and Li, J.Z., Clinic experimentation diagnostics, Beijing: People Sanitation Press, 1989, p. 425, p. 674.
Sanz, V., Marcos, S.D., and Galbán, J. Anal. Chim. Acta, 2008, vol. 607, no. 2, p. 211.
Xing, X.P., Shi, X., Zhang, M.J., Jin, W., and Ye, J.N., Chromatographia, 2008, vol. 67, no. 11/12, p. 985.
Jen, J.F., Hsiao, S.L., and Liu, K.H., Talanta, 2002, vol. 58, no. 4, p. 711.
Dutra, R.F., Moreira, K.A., Oliveira, M.I.P., Araújo, A.N., Montenegro, M.C.B.S., Filho, J.L.L., and Silva, V.L., Electroanal., 2005, vol. 17, no. 8, p. 701.
Liu, A., Honma, I., and Zhou H., Biosens. Bioelectron, 2007, vol. 23, no. 1, p. 74.
Kasai, S., Hirano, Y., Motochi, N., Shiku, H., Nishizawa, M., and Matsue, T., Anal. Chim. Acta, 2002, vol. 458, no. 2, p. 263.
Bian, W.W., Zhang, N., and Jiang, C.Q., Luminescence, 2011, vol. 26, no. 1, p. 51.
Author information
Authors and Affiliations
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Bian, W.W., Wang, L., Zhang, N. et al. Spectrofluorimetric method for the determination of uric acid in human serum. J Anal Chem 69, 480–484 (2014). https://doi.org/10.1134/S1061934814050025
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934814050025