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Voltammetric determination of immunosuppressive agent, azathioprine, by using a graphene-chitosan modified-glassy carbon electrode

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Abstract

Electrodeposition of graphene-chitosan (GR-CS) composite onto glassy carbon electrode was done via a simple one-step strategy. Different methods were used for surface characterization which confirmed the presence of a nano-structure of graphene sheets. The modified electrode was highly efficient in azathioprine analysis by square-wave voltammetry. Reversibility of the redox couple of azathioprine was significantly improved at the surface of GR-CS modified electrode compared to the bare electrode. A linear range of 1.0 × 10−7 to 2.6 × 10−5 M of azathioprine was obtained with a sensitivity of 5.54 A/M and a detection limit of 4.7 × 10−8 M. The proposed sensor exhibited high reproducibility (RSD % = 5%) and repeatability (RSD % = 3.5%) and long-term storage stability. The modified electrode was applied successfully to the accurate determination of azathioprine in pharmaceutical and blood serum samples.

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References

  1. Novoselov, K.S., Geim, A.K., Morozov, S.V., Jiang, D., Zhang, Y., Dubonos, S.V., and Grigorieva, I.V., Science, 2004, vol. 306, p. 666.

    Article  CAS  Google Scholar 

  2. Segal, M., Nat. Nano, 2009, vol. 4, p. 612.

    Article  CAS  Google Scholar 

  3. Allen, M.J., Tung, V.C., and Kaner, R.B., Chem. Rev., 2010, vol. 110, p. 132.

    Article  CAS  Google Scholar 

  4. Choi, W., Lahiri, I., Seelaboyina, R., and Kang, Y.S., Crit. Rev. Solid State, 2010, vol. 35, p. 52.

    Article  CAS  Google Scholar 

  5. Shao, Y., Wang, J., Wu, H., Liu, J., Aksay, I.A., and Lin Y., Electroanalysis, 2010, vol. 22, p. 1027.

    Article  CAS  Google Scholar 

  6. Tkac, J., Whittaker, J.W., and Ruzgas, T., Biosens. Bioelectron., 2007, vol. 22, p. 1820.

    Article  CAS  Google Scholar 

  7. Kaushik, A., Solanki, P.R., Ansari, A.A., Ahmad, S., and Malhotra, B.D., Electrochem. Commun., 2008, vol. 10, p. 1364.

    Article  CAS  Google Scholar 

  8. Khan, R. and Dhayal, M., Electrochem. Commun., 2008, vol. 10, p. 263.

    Article  CAS  Google Scholar 

  9. Xi, F., Liu, L., and Wu, Q., Biosens. Bioelectron., 2008, vol. 24, p. 29.

    Article  CAS  Google Scholar 

  10. Lin, M.S. and Len, H.J., Electroanalysis, 2005, vol. 17, p. 2068.

    Article  CAS  Google Scholar 

  11. Pumera, M., Ambrosi, A., Bonanni, A., Chng, E.L.K., and Poh, H.L., TrAC Trends Analyt. Chem., 2010, vol. 29, p. 954.

    Article  CAS  Google Scholar 

  12. Yang, S., Luo, S., Liu, C., and Wei, W., Coll. Surf., Ser. B, 2012, vol. 96, p. 75.

    Article  CAS  Google Scholar 

  13. Simmons, R., Canafax, D., Fryd, D., Ascher, N., Payne, W., Sutherland, D., et al., Transplant. Proc., 1986, vol. 18(Suppl. 1), p. 76.

    CAS  Google Scholar 

  14. Nashel, D., Med. Clin. North Am., 1985, vol. 69, p. 817.

    CAS  Google Scholar 

  15. Fox, D. and McCune, W., Concepts Immunopathol., 1989, vol. 7, p. 20.

    CAS  Google Scholar 

  16. Counihan, T. and Feighery, C., Ir. J. Med. Sci., 1991, vol. 160, p. 199.

    Article  CAS  Google Scholar 

  17. Ho, V. and Zloty, D., Dermatol. Clin., 1993, vol. 11, p. 73.

    Article  CAS  Google Scholar 

  18. Dervieux, T. and Boulieu, R., Clin. Chem., 1998, vol. 44, p. 551.

    CAS  Google Scholar 

  19. Mueller, X., Ann. Thorac. Surg., 2004, vol. 77, p. 354.

    Article  Google Scholar 

  20. Lakshmi, C.S.R. and Reddy, M.N., Talanta, 1998, vol. 47, p. 1279.

    Article  CAS  Google Scholar 

  21. Bhaskar, M., Manohara, Y., Gayasuddin, M., Balaraju, M., and Kumar, T.B., Int. J. Chem. Tech. Res., 2010, vol. 2, p. 376.

    CAS  Google Scholar 

  22. Binscheck, T., Meyer, H., and Wellhöner, H.H., J. Chromatogr., Ser. B, 1996, vol. 675, p. 287.

    Article  CAS  Google Scholar 

  23. Fazio, T.T., Singh, A.K., Kedor-Hackmann, E.R.M., and Santoro, M.I.R.M., J. Pharmaceut. Biomed., 2007, vol. 43, p. 1495.

    Article  CAS  Google Scholar 

  24. Sastry, C.P., Srinivas, K., and Prasad, K.M.K., Microchim. Acta, 1996, vol. 122, p. 77.

    Article  CAS  Google Scholar 

  25. Göger, N.G., Parlatan, H.K., Basan, H., Berkkan, A., and Özden, T., J. Pharmaceut. Biomed., 1999, vol. 21, p. 685.

    Article  Google Scholar 

  26. Uslu, B. and Ozkan, S.A., Anal. Lett., 2007, vol. 40, p. 817.

    Article  CAS  Google Scholar 

  27. Xu, Q., Yuan, A.-J., Zhang, R., Bian, X., Chen, D., and Hu, X., Curr. Pharm. Anal., 2009, vol. 5, p. 144.

    Article  Google Scholar 

  28. Shahrokhian, S. and Ghalkhani, M., Electrochem. Commun., 2009, vol. 11, p. 1425.

    Article  CAS  Google Scholar 

  29. Shahrokhian, S. and Ghalkhani, M., Electrochim. Acta, 2010, vol. 55, p. 3621.

    Article  CAS  Google Scholar 

  30. Hummers, W.S. and Offeman, R.E., J. Am. Chem. Soc., 1958, vol. 80, p. 1339.

    Article  CAS  Google Scholar 

  31. Xu, Y., Bai, H., Lu, G., Li, C., and Shi, G., J. Am. Chem. Soc., 2008, vol. 130, p. 5856.

    Article  CAS  Google Scholar 

  32. Stankovich, S., Dikin, D.A., Piner, R.D., Kohlhaas, K.A., Kleinhammes, A., Jia, Y., et al., Carbon, 2007, vol. 45, p. 1558.

    Article  CAS  Google Scholar 

  33. Kang, X., Wang, J., Wu, H., Liu, J., Aksay, I.A., and Lin, Y., Talanta, 2010, vol. 81, p. 754.

    Article  CAS  Google Scholar 

  34. Kochmann, S., Hircsh, T., and Wolfbeis, O.S., TrAC Trends Analyt. Chem., 2012, vol. 39, p. 87.

    Article  CAS  Google Scholar 

  35. Bard, A.J. and Faulkner, L.R., Electrochemical Methods Fundamentals and Applications, New York: John Wiley & Sons, 2001.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Razi University, 67346, Kermanshah, Iran

    Zahra Hatami & Fahimeh Jalali

Authors
  1. Zahra Hatami
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  2. Fahimeh Jalali
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Correspondence to Fahimeh Jalali.

Additional information

Published in Russian in Elektrokhimiya, 2015, Vol. 51, No. 1, pp. 81–88.

The article is published in the original.

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Hatami, Z., Jalali, F. Voltammetric determination of immunosuppressive agent, azathioprine, by using a graphene-chitosan modified-glassy carbon electrode. Russ J Electrochem 51, 70–76 (2015). https://doi.org/10.1134/S1023193515010097

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  • DOI: https://doi.org/10.1134/S1023193515010097

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