Abstract
For the first time, genetic characteristics of 15 local populations of eight ethnic groups of Eastern Europe were obtained using an expanded panel that included 21 autosomal STR markers used in forensic practice for DNA identification and kinship establishment. The assessment of polymorphism of markers in the Russian, Belarusian, and Moldovan populations was carried out, and the levels of intra- and interpopulation genetic differentiation of the studied population groups were characterized. The parameters of the informational significance of the system of autosomal microsatellites for expert DNA identification and establishment of kinship were estimated. A database of allele frequencies of autosomal STR loci has been developed, which can be used to carry out probabilistic and statistical calculations when assessing the level of reliability of an expert study in the countries of the Union State. It was shown that population genetic structure should be taking into account in forensic DNA analysis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Szczerkowska, Z., Kapińska, E., Wysocka, J., and Cybulska, L., Northern Polish population data and forensic usefulness of 15 autosomal STR loci, Forensic Sci. Int., 2004, vol. 144, no. 1, pp. 69–71. https://doi.org/10.1016/j.forsciint.2004.02.020
Jacewicz, R., Jedrzejczyk, M., Ludwikowska, M., and Berent, J., Population database on 15 autosomal STR loci in 1000 unrelated individuals from the Lodz region of Poland, Forensic Sci. Int. Genet., 2008, vol. 2, no. 1, pp. e1–e3. https://doi.org/10.1016/j.fsigen.2007.08.002
Malyarchuk, B.A., Woźniak, M., Czarny, J., et al., Variation of 15 autosomal microsatellite DNA loci in the Russian population, Mol. Biol. (Moscow), 2007, vol. 41, no. 1, pp. 1–4. https://doi.org/10.1134/S0026893307010013
Piatek, J., Jacewicz, R., Ossowski, A., et al., Population genetics of 15 autosomal STR loci in the population of Pomorze Zachodnie (NW Poland), Forensic Sci. Int. Genet., 2008, vol. 2, no. 3, pp. e41–e43. https://doi.org/10.1016/j.fsigen.2007.09.002
Rebała, K., Wysocka, J., Kapińska, E., et al., Belarusian population genetic database for 15 autosomal STR loci, Forensic Sci. Int., 2007, vol. 173, nos. 2–3, pp. 235–237. https://doi.org/10.1016/j.forsciint.2007.02.002
Pepiński, W., Skawrońska, M., Niemcunowicz-Janica, A., et al., Polymorphism of 11 autosomal STRs in a population sample of Belarussian minority residing in the region of Podlasie (Northeastern Poland)—an extension of the STR core set, Arch. Med. Sadowej Kryminol., 2008, vol. 58, nos. 2–3, pp. 65–68.
Zhivotovsky, L.A., Malyarchuk, B.A., Derenko, M.V., et al., Developing STR databases on structured populations: the native South Siberian population versus the Russian population, Forensic Sci. Int. Genet., 2009, vol. 3, no. 4, pp. e111–e116. https://doi.org/10.1016/j.fsigen.2008.08.001
Parys-Proszek, A., Kupiec, T., Wolańska-Nowak, P., and Branicki, W., Genetic variation of 15 autosomal STR loci in a population sample from Poland, Leg. Med. (Tokyo), 2010, vol. 12, no. 5, pp. 246–248. https://doi.org/10.1016/j.legalmed.2010.05.002
Stepanov, V.A., Melnikov, A.V., Lash-Zavada, A.Y., et al., Genetic variability of 15 autosomal STR loci in Russian populations, Leg. Med. (Tokyo), 2010, vol. 12, no. 5, pp. 256–258. https://doi.org/10.1016/j.legalmed.2010.05.006
Stepanov, V.A., Balanovsky, O.P., Melnikov, A.V., et al., Characteristics of populations of the Russian Federation over the panel of fifteen loci used for DNA identification and in forensic medical examination, Acta Nat., 2011, vol. 3, no. 2, pp. 59–71. https://doi.org/10.32607/20758251-2011-3-2-56-67
Soltyszewski, I., Pepinski, W., Wolanska-Nowak, P., et al., Polish population data on 15 autosomal STRs of AmpFlSTR NGM PCR kit, Forensic Sci. Int. Genet., 2014, vol. 9, pp. 142–149. https://doi.org/10.1016/j.fsigen.2013.12.002
Tsybovskii, I.S., Veremeichik, V.M., Kotova, S.A., et al., Developing forensic reference database by 18 autosomal STR for DNA identification in Republic of Belarus, Russ. J. Genet., 2017, vol. 53, no. 2, pp. 275–284. https://doi.org/10.1134/S1022795417020132
Benvisto, A., Messina, F., Finocchio, A., et al., A genetic portrait of the South-Eastern Carpathians based on autosomal short tandem repeats loci used in forensics, Am. J. Hum. Biol., 2018, vol. 30, no. 5. e23139. https://doi.org/10.1002/ajhb.23139
Rębała, K., Veselinović, I., Siváková, D., et al., Northern Slavs from Serbia do not show a founder effect at autosomal and Y-chromosomal STRs and retain their paternal genetic heritage, Forensic Sci. Int. Genet., 2009, vol. 3, no. 4, pp. e133–e136. https://doi.org/10.1016/j.fsigen.2008.11.001
Excoffier, L., Laval, G., and Schneider, S., Arlequin ver. 3.0: an integrated software package for population genetics data analysis, Evol. Bioinf. Online, 2005, vol. 1, pp. 47–50.
Desmarais, D., Zhong, Y., Chakraborty, R., et al., Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA), J. Forensic Sci., 1998, vol. 43, pp. 1046–1049.
Funding
This work was carried out within the framework of the Scientific and Technical Program of the Union State “Development of Innovative Genogeographic and Genomic Technologies for Identification of a Person and Individual Characteristics of a Person on the Basis of the Study of Gene Pools of Regions of the Union State” (“DNA Identification”), state contract no. 011-17 dated September 26, 2017.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest. The authors declare that they have no conflict of interest.
Statement of compliance with standards of research involving humans as subjects. All procedures performed in a study involving people comply with the ethical standards of the institutional and/or national committee for research ethics and the 1964 Helsinki Declaration and its subsequent changes or comparable ethical standards.
Informed voluntary consent was obtained from each of the participants.
Supplementary Information
Rights and permissions
About this article
Cite this article
Kharkov, V.N., Kotova, S.A., Kolesnikov, N.A. et al. Genetic Diversity of 21 Autosomic STR Markers of the CODIS System in Populations of Eastern Europe. Russ J Genet 57, 1408–1413 (2021). https://doi.org/10.1134/S102279542112005X
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S102279542112005X