Abstract
Egypt is an important geographical location for wintering puddle ducks. In order to investigate their relationships, 30 ducks from genus Anas were sampled from different aquatic regions of New Damietta, Damietta Governorate, Egypt. Many phylogenetic studies used mitochondrial cytochrome c oxidase subunit I (COI) as an effective molecular marker. The COI barcodes of six ducks species were analyzed in this work from one genus Anas belonging to family Anatidae. The studied species of ducks were identified correctly from their DNA barcodes. The distances were calculated between barcodes by utilizing of utilizing Kimura two parameter (K2P). Maximum Likelihood was used to construct a phylogenetic tree, which grouped the entire genus into three divergent clades. Within family Anatidae, COI analysis supported a basal position of Anas clypeata and Anas querquedula. It also supported the sister group relationship between Anas carolinensis and Anas platyrhynchos and the cluster comprising Anas acuta and Anas strepera. It was concluded that most Anatidae species have distinct COI sequences. DNA barcoding is a powerful molecular tool for species identification and phylogenetic inference of Anatidae.
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Deef, L.E. Phylogenetic Relationships among Puddle Duck Species in Egypt using COI Gene Variations in mtDNA. Russ J Genet 57, 1189–1197 (2021). https://doi.org/10.1134/S1022795421100045
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DOI: https://doi.org/10.1134/S1022795421100045