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Content of Gentiopicroside in Natural Populations of Gentiana cruciata L., Growing in the Territory of the Republic of Tatarstan

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Abstract

This report contains the results of quantitative analysis of primary iridoid gentiopicroside, phenolic compounds, and flavonoids in the shoots, rhizomes, and roots of cross gentian (Gentiana cruciata L.) plants collected in natural phytocenoses of Alekseevskii, Zelenodol’skii, and Apastovskii raions in the Republic of Tatarstan. The content of phenolic compounds and flavonoids in herbs was determined spectrophotometrically. It was shown that the greatest content of phenolic compounds and flavonoids in above-ground parts was 19.4 and 10.80 mg/g in Zelenodol’skii and Alekseevskii raions and that in the rhizomes and roots was 8.53 and 1.96 mg/g in Apastovskii raion. By the method of high-performance liquid chromatography, it was shown that the greatest quantity of gentiopicroside (more than 9%) was accumulated in the underground part of plants, whereas the above-ground part contained approximately 6% of this compound. Thus, cross gentian growing in plant communities located in these raions differed in the content of biologically active substances, which probably depended on the joint effect of ecological and cenotic characteristics of habitats. The results of phytochemical analysis suggest that Apastovskii raion in the Republic of Tatarstan may be a promising locality for picking and preparing medicinal herbs with a high content of main iridoid gentiopicroside.

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Funding

This work was supported by the Program of Strategic Academic Leadership, Kazan Federal University (PRIORI-TET-2030) and supported in part by the state assignment given to Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    L. Z. Khusnetdinova, S. A. Dubrovnaya & O. A. Timofeeva

  2. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    A. N. Akulov

  3. Kazan State University of Architecture and Engineering, Kazan, Russia

    R. M. Mukhametshina

Authors
  1. L. Z. Khusnetdinova
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  2. A. N. Akulov
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  3. S. A. Dubrovnaya
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  4. R. M. Mukhametshina
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  5. O. A. Timofeeva
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Correspondence to L. Z. Khusnetdinova.

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Translated by N. Balakshina

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Abbreviations: MPRM medicinal plant raw materials; RT—Republic of Tatarstan.

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Khusnetdinova, L.Z., Akulov, A.N., Dubrovnaya, S.A. et al. Content of Gentiopicroside in Natural Populations of Gentiana cruciata L., Growing in the Territory of the Republic of Tatarstan. Russ J Plant Physiol 70, 162 (2023). https://doi.org/10.1134/S1021443723700218

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  • DOI: https://doi.org/10.1134/S1021443723700218

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