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Intraspecific Photosynthetic Diversity and Differences in Stress-Induced Plasticity in С3–С4 Sedobassia sedoides under Drought Stress

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Abstract

Research into organization and peculiarities of carbon-concentrating mechanism (CCM) functioning in plants with intermediate C3–С4 (C2) photosynthesis type characterized by resistance to arid climate is of paramount importance given the global climate changes. In this study two populations (P1 and P2) of С3–С4 Sedobassia sedoides (Chenopodiaceae) different in productivity were investigated under PEG-induced osmotic stress. As a result of our studies, a less productive P1 population was classified as the Type II of С2 photosynthesis, since there was a relatively low efficiency of cyclic electron transport around PSI, and high glycine decarboxylase (GDC) content. On the contrary, P2 population was more productive and based on type of photosynthesis was classified as С4-like plants. It exhibited a higher efficiency of cyclic electron transport around PSI and lower GDC content. Plants in each population also demonstrated heterogeneity in terms of the degree of dehydration under osmotic stress and fell into two groups: stress-tolerant (ST) and not stress-tolerant (NST) plants. NST plants from both populations showed a significant increase in proline content and a decrease of maximum quantum yield of PSII (Fv/Fm) evidencing a severe osmotic stress. P1 and P2 plants showed differences in mechanisms ensuring drought tolerance, more pronounced in NST plants. NST plants from P1 showed a decrease in K+/Na+ ratio, an increase in cyclic electron flow around PSI and a decrease in efficiency of PSII (due to NPQ), presence of oxidative stress (an increase in catalase and SOD activity), increase in content of C4 CCM enzymes PEPС and NAD-Me. NST plants from P2 retained considerable dry biomass and shoot length (which were formed before stress), demonstrated a decline in Na+ accumulation, an increase in K+/Na+ ratio, and a decrease in activity of cyclic electron flow around PSI and PSII efficiency (due to NPQ), absence of oxidative stress, a decrease in Rubisco content and content of C4 CCM enzymes PEPC and NAD-Me. The complexity of determining the specific mechanisms of biochemical response of plants to stress is discussed, as well as the high stress-induced plasticity of this type of photosynthesis, i.e. the possibility of switch from one type of C3–C4 photosynthesis to another under drought, either towards enhancing С4 characteristics or towards their weakening.

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Funding

The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme no.: 122042700044-6).

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  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276, Moscow, Russia

    Z. F. Rakhmankulova, E. V. Shuyskaya & M. Yu. Prokofieva

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  1. Z. F. Rakhmankulova
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Contributions

Authors ZFR and EVS designed the experiments. ZFR, EVS, and MYuP collected samples and performed the experiments. ZFR drafted the manuscript and all authors revised it.

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Correspondence to Z. F. Rakhmankulova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants as objects of research.

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Rakhmankulova, Z.F., Shuyskaya, E.V. & Prokofieva, M.Y. Intraspecific Photosynthetic Diversity and Differences in Stress-Induced Plasticity in С3–С4 Sedobassia sedoides under Drought Stress. Russ J Plant Physiol 70, 81 (2023). https://doi.org/10.1134/S1021443722603135

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