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Effect of 24-Epicastasterone and Its Monosalicylate on Salt Resistance of Arabidopsis thaliana Wild Type and the Salicylate-Deficit NahG Transformants

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Abstract

The effects of 24-epicastasterone (EC) and those of its conjugate with a salicylic acid residue—24‑epicastasterone 2-monosalicylate (MSEC)—on the resistance of Arabidopsis thaliana L. plants to 24-h contact with 175 mM NaCl were assessed. The wild type Col-0 plants and those transformed with the bacterial NahG gene of salicylate hydroxylase were compared in this respect. The pretreatment of the wild plants with 0.01–1 µM EC elevated their salt resistance, which was expressed as the decrease in their LPO rate, stabilization of the chlorophyll pool, and increase in the carotenoid level after the plants’ exposure to NaCl. MSEC also afforded a protective and more pronounced effect on the Col-0 plants. In the salt-stressed wild type plants, EC enhanced the activities of antioxidant enzymes catalase and guaiacol peroxidase; the treatment with MSEC significantly activated superoxide dismutase and catalase. Meanwhile, neither EC nor MSEC influenced the activities of the antioxidant enzymes in the NahG plants that are unable to accumulate salicylic acid. In addition, neither EC nor MSEC changed the LPO intensity and the level of photosynthetic enzymes under salt stress. It was inferred that salicylic acid is involved in the mechanism of the protective action of brassinosteroids against consequences of the strong salinization. MSEC is considered to be a practically prospective agent promoting salt resistance of plants.

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ACKNOWLEDGMENTS

We thank T.O. Yastreb for the valuable methodical consultation.

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, 220141, Minsk, Belarus

    R. P. Litvinovskaya & V. A. Khripach

  2. Dokuchaev National Agrarian University, 62483, Kharkiv, Ukraine

    M. A. Shkliarevskyi, Yu. E. Kolupaev & A. I. Kokorev

  3. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 03143, Kyiv, Ukraine

    A. P. Dmitriev

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  1. R. P. Litvinovskaya
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  2. M. A. Shkliarevskyi
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  3. Yu. E. Kolupaev
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Correspondence to Yu. E. Kolupaev.

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Translated by A. Aver’yanov

Abbreviations: BS—brassinosteroids; CAT—catalase; EC—24‑epicastasterone; GPO—guaiacol peroxidase; MSEC—24‑epicastasterone 2-monosalicylate; SA—salicylic acid; SOD superoxide dismutase.

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Litvinovskaya, R.P., Shkliarevskyi, M.A., Kolupaev, Y.E. et al. Effect of 24-Epicastasterone and Its Monosalicylate on Salt Resistance of Arabidopsis thaliana Wild Type and the Salicylate-Deficit NahG Transformants. Russ J Plant Physiol 69, 35 (2022). https://doi.org/10.1134/S1021443722020108

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