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Effects of Nitrate and L-Arginine on Content of Nitric Oxide and Activities of Antioxidant Enzymes in Roots of Wheat Seedlings and Their Heat Resistance

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Abstract

Separate and combined effects of nitrate (NaNO3) and L-arginine as potential sources of nitric oxide (NO) on the content of endogenous NO in roots of wheat (Triticum aestivum L.) seedlings and on their heat resistance were studied. Both agents increased the seedling resistance to the damaging heating; the effect was maximal at 20 mM NaNO3 or 5 mM L-arginine. The treatment with L-arginine elevated the NO content in the roots within the first 2 h of the treatment. Nitrate caused a stronger and longer rise in nitric oxide. Activity of nitrate reductase considerably (2–3 times) increased in the roots exposed to nitrate. The augmentation in the nitric oxide level caused by nitrate or L-arginine was prevented by the root pretreatment with an inhibitor of nitrate reductase (sodium tungstate) or an inhibitor of animal NO-synthase—NG-nitro-L-arginine methyl ester (L-NAME). Upon the combined treatment with NaNO3 and L-arginine, the nitrateinduced stimulation of the nitrate reductase activity, NO level in the roots, and seedling heat resistance were less pronounced than after separate application. In the presence of L-NAME, the negative influence of L-arginine on nitrate effects was markedly attenuated. The plant exposure to nitrate or L-arginine increased the activities of antioxidant enzymes (superoxide dismutase, catalase, and guaiacol peroxidase). A mixture of NaNO3, and L-arginine caused weaker effects. It was suggested that nitrate-dependent and arginine-dependent pathways of NO formation are antagonistic to each other in wheat roots.

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Abbreviations

CAT:

catalase

GPO:

guaiacol peroxidase

L-NAME:

NG-nitro-L-arginine methyl ester (inhibitor of NO-synthase)

NR:

nitrate reductase

PTIO:

2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (scavenger of NO)

SOD:

superoxide dismutase

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Authors and Affiliations

  1. Dokuchaev National Agrarian University, Kharkiv, 62483, Ukraine

    Yu. V. Karpets, Yu. E. Kolupaev, A. A. Lugovaya, N. V. Shvidenko & T. O. Yastreb

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  1. Yu. V. Karpets
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  2. Yu. E. Kolupaev
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  3. A. A. Lugovaya
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  4. N. V. Shvidenko
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  5. T. O. Yastreb
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Correspondence to Yu. E. Kolupaev.

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Original Russian Text © Yu.V. Karpets, Yu.E. Kolupaev, A.A. Lugovaya, N.V. Shvidenko, T.O. Yastreb, 2018, published in Fiziologiya Rastenii, 2018, Vol. 65, No. 6, pp. 472–480.

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Karpets, Y.V., Kolupaev, Y.E., Lugovaya, A.A. et al. Effects of Nitrate and L-Arginine on Content of Nitric Oxide and Activities of Antioxidant Enzymes in Roots of Wheat Seedlings and Their Heat Resistance. Russ J Plant Physiol 65, 908–915 (2018). https://doi.org/10.1134/S1021443718050096

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  • DOI: https://doi.org/10.1134/S1021443718050096

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