Abstract
The paper is devoted to characterization of BEA and MFI zeolite samples synthesized in alkaline and fluoride media. Using a number of synthesized and commercial samples, enzyme adsorption isotherms were recorded, specifically for Aspergillus oryzae β-galactosidase. The highest adsorption capacity (32–33 mg/g) was achieved on fine-crystalline (0.2–0.6 μm) MFI samples. The fine-crystalline BEA samples (0.3–1 and ˂0.1 μm) exhibited maximal adsorption capacities of 18 and 26 mg/g, respectively. The coarse-crystalline BEA and MFI zeolites prepared by fluoride synthesis reached a markedly lower enzyme adsorption capacity, not exceeding 6 mg/g. The NMR spectroscopy data indicate that the low adsorption capacity of the zeolites synthesized in fluoride media was not only owing to their crystal size but also to the low content of hydroxyl groups on their surface.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Harju, M., Kallioinen, H., and Tossavainen, O., Int. Dairy J., 2012, vol. 22, pp. 104–109. https://doi.org/10.1016/j.idairyj.2011.09.011
Kuribayashi, L.M., do Rio Ribeiro, V.P., de Santana, R.C., Ribeiro, E.J., dos Santos, M.G., Falleiros, L.N.S.S., and Guidini, C.Z., Appl. Microbiol. Biotechnol., 2021, vol. 105, pp. 3601–3610. https://doi.org/10.1007/s00253-021-11325-8
Zhang, H., Jiang, Z., Xia, Q., and Zhou, D., Biochem. Eng. J., 2021, vol. 172, p. 108033. https://doi.org/10.1016/j.bej.2021.108033
Poletto, M., Parascandola, P., Saracino, I., and Cifarelli, G., Int. J. Chem. React. Eng., 2005, vol. 3, no. 1. https://doi.org/10.2202/1542-6580.1275
Corma, A., Fornés, V., Jordá, J.L., Rey, F., FernandezLafuente, R., Guisan, J.M., and Mateo, C., Chem. Commun., 2001, pp. 419–420. https://doi.org/10.1039/b009232k
Tavolaro, A., Tavolaro, P., and Drioli, E., Colloids Surf., B, 2007, vol. 55, pp. 67–76. https://doi.org/10.1016/j.colsurfb.2006.11.010
Wu, J., Li, X., Yan, Y., Hu, Y., Zhang, Y., and Tang, Y., J. Colloid. Interface Sci., 2013, vol. 406, pp. 130–138. https://doi.org/10.1016/j.jcis.2013.05.073
Atyaksheva, L.F., Dobryakova, I.V., Kostyukov, I.A., and Kolyagin, Yu.G., Petrol. Chem., 2022, vol. 62, no. 3, pp. 316–321. https://doi.org/10.1134/S0965544122030021
Matsui, M., Kiyozumi, Y., Mizushina, Y., Sakaguchi, K., and Mizukami, F., Sep. Purif. Technol., 2015, vol. 149, pp. 103–109. https://doi.org/10.1016/j.seppur.2015.05.023
Camblor, M.A., Corma, A., and Valencia, S., J. Mater. Chem., 1998, vol. 8, pp. 2137–2145. https://doi.org/10.1039/A804457K
Pavlov, V.S., Bruter, D.V., Konnov, S.V, and Ivanova, I.I., Petrol. Chem., 2020, vol. 60, no. 8, pp. 929–936. https://doi.org/10.1134/S0965544120080095
Ivanova, I.I. and Knyazeva, E.E., RF Patent 2640236, 2017
Tanaka, Y., Kagamiishi, A., Kiuchi, A., and Horiuchi, T., J. Biochem., 1975, vol. 77, pp. 241–247. https://doi.org/10.1093/oxfordjournals.jbchem.a130713
Atyaksheva, L.F., Fedosov, D.A., Ivanova, M.V., Kasyanov, I.A., Kolozhvari, B.A., and Ivanova, I.I., Russ. J. Phys. Chem. A, 2018, vol. 92, no. 9, pp. 1846–1850. https://doi.org/10.1134/S0036024418090042
Chukhrai, E.S., Atyaksheva, L.F., and Pilipenko, O.S., Russ. J. Phys. Chem. A, 2011, vol. 85, no. 5, pp. 888–894. https://doi.org/10.1134/S0036024411050086
Funding
The study was financially supported by the Russian Science Foundation (RSF Grant no. 20-13-00203).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare no conflict of interest requiring disclosure in this article.
Rights and permissions
About this article
Cite this article
Atyaksheva, L.F., Andriako, E.P., Bachurina, D.O. et al. Effects of the Synthesis Conditions and Structural Characteristics of Zeolites on the Adsorption of β-Galactosidase. Pet. Chem. 62, 980–987 (2022). https://doi.org/10.1134/S0965544122070040
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0965544122070040