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Multicomponent Analysis of Biomarkers in Exhaled Air Using Diode Laser Spectroscopy

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Abstract

We have proposed methods of multicomponent spectral analysis of exhaled air based on the use of tunable diode lasers to solve some problems in biomedical diagnostics. Simultaneous laser spectral analysis of CO and CO2, CO and N2O pairs near 4.7 µm can be used to examine gas exchange in the studies on the physiology of respiration and cardiovascular diagnostics conducted using various stress tests and for solving problems of anesthesiology and continuous monitoring ventilation–perfusion status. Simultaneous analysis of NO and CO2 at approximately 5.4 µm can be useful for monitoring the breathing maneuver in studies of inflammatory processes in the distal lungs. Simultaneous detection of NH3, CO2, and C2H4 near 10.5 µm is promising for studies of general metabolism and major metabolic cycles. Simultaneous analysis of 13CO2 and 12CO2 near 2.05 µm can be used to measure the ratio of 13CO2 to 12CO2 when conducting isotopic breathing tests. The results of the analysis of promising spectral ranges and the relative position of the analytical lines in them are given. The possibility of simultaneous detection of several molecules under study in the proposed spectral regions was experimentally demonstrated, and laser transmission spectra were obtained. The proposed approach can be applied to the analysis of the microcomposition of exhaled air.

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Funding

This work was supported as a part of a research program planned at the Prokhorov General Physics Institute of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Prokhorov General Physics Institute, Russian Academy of Sciences, 119991, Moscow, Russia

    E. V. Stepanov & S. G. Kasoev

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  1. E. V. Stepanov
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  2. S. G. Kasoev
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Correspondence to E. V. Stepanov.

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Translated by O. Zhukova

The 22nd Annual Conference Saratov Fall Meeting 2018 (SFM’18): VI International Symposium “Optics and Biophotonics” and XXII International School for Junior Scientists and Students on Optics, Laser Physics, and Biophotonics, September 24−29, 2018, Saratov, Russia. https://www.sgu.ru/structure/fiz/saratov-fall-meeting/previousconferences/saratov-fall-meeting-2018

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Stepanov, E.V., Kasoev, S.G. Multicomponent Analysis of Biomarkers in Exhaled Air Using Diode Laser Spectroscopy. Opt. Spectrosc. 126, 736–744 (2019). https://doi.org/10.1134/S0030400X19060249

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