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Overexpression of MKRN2 Inhibits the Growth of Ovarian Cancer Cells

  • MOLECULAR BIOLOGY OF THE CELL
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Abstract

Ovarian cancer has a high mortality with low five-year survival rates. The role of the E3 ligase Makorin ring finger protein 2 (MKRN2) in ovarian cancer is unknown. This study investigated the impact of MKRN2 on the growth of ovarian cancer. MKRN2 expression in ovarian cancer tissue was analyzed by immunohistochemistry. Overexpression of MKRN2 was induced in two ovarian cancer cell lines (SKOV3 and CAOV3) by lentivirus transfection, and expression levels were verified by western blotting. Proliferation and growth were determined by CCK-8 and colony formation assays, while migration was examined using transwell assays and apoptosis by flow cytometry. Xenograft tumors of transfected SKOV3 cells were established in mice, and immunohistochemistry and TUNEL assays measured MKRN2 levels and apoptosis in tumor cells. Reduced levels of MKRN2 in cancerous tissue relative to non-cancerous ovarian tissues. Lentiviral-based MKRN2 overexpression in SKOV3 and CAOV3 cells reduced tumor-associated behavior while inducing apoptosis in vitro. In xenograft tumors, MKRN2 overexpression inhibited ovarian cancer growth and increased apoptosis in vivo. These findings imply the MKRN2 involvement in ovarian carcinogenesis and suggest its potential for treating the disease.

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ADDITIONAL INFORMATION

The text was submitted by the author(s) in English.

Funding

The study is funded by the National Natural Science Foundation of China (no. 81302251) and the Suzhou Science and Education Project for Youth scholars (JKXW2018078).

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Dushu Lake Hospital affiliated to Soochow University, Soochow University, Suzhou, China

    F. Z. Jiang & Y. M. Zhang

  2. Obstetrics and Gynecology Department, Nantong Haimen District People’s Hospital, Nantong, China

    Q. J. Xia

  3. Suzhou Industrial Park Centers for Disease Control and Prevention, Suzhou, China

    L. Wu

Authors
  1. F. Z. Jiang
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  2. Q. J. Xia
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  3. L. Wu
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  4. Y. M. Zhang
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Contributions

Feizhou Jiang and Qinjian Xia have contributed equally to this work. Feizhou Jiang and Qinjian Xia designed the study, conducted experimental studies, and completed the manuscript. Lei Wu participated in the experimental studies and data analysis. Yueming Zhang supervised the work and reviewed the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Y. M. Zhang.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. In carrying out this work, all ethical standards were observed. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

This article does not contain any studies involving human participants performed by any authors.

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Jiang, F.Z., Xia, Q.J., Wu, L. et al. Overexpression of MKRN2 Inhibits the Growth of Ovarian Cancer Cells. Mol Biol 57, 684–691 (2023). https://doi.org/10.1134/S002689332304009X

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  • DOI: https://doi.org/10.1134/S002689332304009X

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