Abstract
Whole-transcriptome data were used to study the changes in expression of genes coding proteins involved in the calcium regulation processes in the hippocampus of male mice with symptoms of depression caused by chronic social defeat stress. Cacna1g, Cacnb3, Camk1g, Camk2d, Camk2n2, Caly, Caln1, S100a16, and Slc24a4 genes were upregulated in the hippocampus of depressed mice compared to a control, while C-acna2d1, Cacng5, Grin2a, and Calm2 were downregulated. The greatest number of significant correlations was observed between the expression level of Calm2, which showed the highest transcriptional activity, and other differentially expressed genes. Calcium signaling in the hippocampus was assumed to be disrupted in mice exposed to chronic social defeat stress. The involvement of Calm2, Сamk1g, Camk2d, and Camk2n2 genes in the process is discussed.
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Funding
This work was supported by State Program 47 “Scientific and Technological Development of the Russian Federation” (2019–2030) (project no. 0134-2019-0002) an agreement with the Institute of Cytology and Genetics SB RAS (project no. FWNR-2022-0019).
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Statement on the welfare of animals. All experiments with mice were performed in compliance with international guidelines for animal research (Directive 2010/63/EU of the European Parliament and of the Council on the Protection of Animals Used for Scientific Purposes). The study protocol (no. 9) was approved by the Ethics Committee at the Institute of Cytology and Genetics SB RAS (Minutes no. 613 dated March 24, 2010).
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Translated by T. Tkacheva
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Pavlova, M.B., Smagin, D.A., Kudryavtseva, N.N. et al. Changes in Expression of Genes Associated with Calcium Processes in the Hippocampus in Mice Exposed to Chronic Social Stress. Mol Biol 57, 356–365 (2023). https://doi.org/10.1134/S0026893323020176
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DOI: https://doi.org/10.1134/S0026893323020176