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Microarray for Quantitative Determination of Inflammatory Biomarkers in a Culture Medium

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Abstract

Cytokines and acute phase proteins play an important role in the development of the immune response during inflammatory reactions. Depending on the type of disease, the development of inflammation is accompanied by changes in concentrations (both decrease and increase) of not one, but many inflammatory biomarkers. Here, a quantitative microarray-based method for multiplex immunoassay of eight biomarkers of human inflammation, namely acute phase proteins (C-reactive protein, serum amyloid protein A) and cytokines (IL-6, IL-8, IL-17, IL-18, IP10/CXCL10, TNFα) was developed and the possibility of its use for the detection of inflammatory biomarkers in a culture medium has been demonstrated. The developed method can be used to evaluate changes of the inflammatory biomarker profile induced by different agents or to determine the concentrations of biomarkers after activation of cells while studying different diseases with the help of in vitro models.

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Funding

The study was financed by the Russian Science Foundation (project no. 19-15-00283).

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  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    S. A. Voloshin, G. U. Feyzkhanova, E. N. Savvateeva, O. V. Smoldovskaya & A. Yu. Rubina

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  1. S. A. Voloshin
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  2. G. U. Feyzkhanova
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  3. E. N. Savvateeva
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  4. O. V. Smoldovskaya
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Correspondence to A. Yu. Rubina.

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The study does not contain any studies performed using biological materials.

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Abbreviations: CRP, C-reactive protein; FBS, fetal bovine serum; IL, interleukin; IP10/CXCL10, interferon-γ-induced protein-10/CXC-chemokine-10; SAA, serum amyloid A protein; TNFα, tumor necrosis factor α; RPMI-1640, Roswell Park Memorial Institute medium 1640.

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Voloshin, S.A., Feyzkhanova, G.U., Savvateeva, E.N. et al. Microarray for Quantitative Determination of Inflammatory Biomarkers in a Culture Medium. Mol Biol 54, 919–928 (2020). https://doi.org/10.1134/S0026893320060138

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  • DOI: https://doi.org/10.1134/S0026893320060138

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