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Functionally Significant Amino Acid Motifs of Heat Shock Proteins: Structural and Bioinformatics Analyses of Hsp60/Hsp10 in Five Classes of Chordata

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Abstract

The Hsp60/Hsp10 chaperonin system is one of the most studied systems of cell emergency responses to stresses associated with changes in environmental conditions. In this regard, we have performed a bioinformatics analysis of 164 amino acid sequences of Hsp60 and 125 amino acid sequences of Hsp10 in five classes of chordata. This enabled uncovering the relationship between the identity of the amino acid composition of Hsp60/Hsp10 and the evolutionary distance between classes of chordata. In the course of the study of the chaperonin crystal structure, potentially significant amino acid motifs responsible for the oligomerization of Hsp60 and Hsp10 monomers and the association/dissociation of the Hsp60 and Hsp10 hetero-dimer have been identified. In addition, we have established that Hsp60 and Hsp10 can form amyloid fibrils due to structural features through the alternative using of the oligomerization sites of monomers as well as association/dissociation sites.

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ACKNOWLEDGMENTS

The authors are grateful to all reviewers for their comments on this paper. The work was supported by the Russian Science Foundation, project no. 18-14-00321.

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  1. Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    T. S. Tikhomirova & O. V. Galzitskaya

  2. Institute for Biological Instrumentation, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia

    T. S. Tikhomirova

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Translated by E. Puchkov

1Abbreviations: Hsp, heat shock protein; MMP, matrix metalloproteinase; Icp55, intermediate cleaving peptidase 55, cleaving the intermediates of posttranslational protein modification; TOM20, translocase of the mitochondrial outer membrane; PKA, protein kinase A; PKG, protein kinase G.

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Tikhomirova, T.S., Galzitskaya, O.V. Functionally Significant Amino Acid Motifs of Heat Shock Proteins: Structural and Bioinformatics Analyses of Hsp60/Hsp10 in Five Classes of Chordata. Mol Biol 52, 761–778 (2018). https://doi.org/10.1134/S0026893318050138

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